Pasick J
Canadian Food Inspection Agency, Retrovirology Centre of Expertise, Centre for Animal and Plant Health, Charlottetown, P.E.I., Canada.
Can J Vet Res. 1998 Oct;62(4):307-10.
Highly purified recombinant gag and env proteins derived from Icelandic strain 1514 of maedi-visna virus were used in an indirect enzyme immunoassay (ELISA) to detect antibodies to small ruminant lentiviruses in sheep and goat sera. The recombinant protein-based ELISA performed very well relative to whole maedi-visna virus and whole caprine arthritis-encephalitis-virus-based ELISAs in its ability to detect anti-maedi visna virus and anti-caprine arthritis-encephalitis virus antibodies, despite the antigenic and genomic variability that is known to exist within and between these two small ruminant lentiviruses. The data suggest that these recombinant maedi-visna virus proteins can be reliably used in an ELISA for the routine serodiagnosis of lentiviral infections in sheep and goats.
源自梅迪-维斯纳病毒冰岛1514株的高度纯化重组gag和env蛋白被用于间接酶免疫测定（ELISA），以检测绵羊和山羊血清中的小反刍兽慢病毒抗体。基于重组蛋白的ELISA在检测抗梅迪-维斯纳病毒和抗山羊关节炎-脑炎病毒抗体方面，相对于基于完整梅迪-维斯纳病毒和完整山羊关节炎-脑炎病毒的ELISA表现良好，尽管已知这两种小反刍兽慢病毒内部和之间存在抗原性和基因组变异性。数据表明，这些重组梅迪-维斯纳病毒蛋白可可靠地用于ELISA，以对绵羊和山羊的慢病毒感染进行常规血清学诊断。
