一种用于检测梅迪-维斯纳病毒抗体的间接酶联免疫吸附测定（ELISA）。
An indirect enzyme-linked immunosorbent assay (ELISA) for the detection of antibodies to maedi-visna virus.
作者信息
Houwers D J, Gielkens A L, Schaake J
出版信息
Vet Microbiol. 1982 Jul;7(3):209-19. doi: 10.1016/0378-1135(82)90035-9.
An indirect enzyme-linked immunosorbent assay (ELISA) for the detection of antibodies to maedi-visna virus (mvv) in sheep is described, in which microtitre plates are sensitized with a partly purified preparation of mvv. The antibodies bound are detected by a horseradish peroxidase conjugate. The results obtained with ELISA on a total of 493 serum samples from several commercial flocks were compared to those of a routine agar gel precipitation test (AGPT) and a complement fixation test (CFT). All samples which scored positive in AGPT, CFT or both (20.8%) were also found positive by ELISA. In addition, with ELISA a further 11.5% of the samples were positive. Serum samples from maedi-free flocks, from sheep suffering from sheep pulmonary adenomatosis and from lambs immunized against other viruses were all negative by ELISA. The assay has been used routinely for some years and proved to be specific, sensitive and suited for screening of large numbers of serum samples.
本文描述了一种用于检测绵羊梅迪-维斯纳病毒（MVV）抗体的间接酶联免疫吸附测定（ELISA）方法，该方法使用部分纯化的MVV制剂对微量滴定板进行包被。通过辣根过氧化物酶偶联物检测结合的抗体。将ELISA法应用于来自几个商业羊群的总共493份血清样本，并与常规琼脂凝胶沉淀试验（AGPT）和补体结合试验（CFT）的结果进行比较。在AGPT、CFT或两者中呈阳性的所有样本（20.8%）通过ELISA检测也呈阳性。此外，ELISA还检测出另外11.5%的样本呈阳性。来自无梅迪羊群、患有绵羊肺腺瘤病的绵羊以及接种过其他病毒疫苗的羔羊的血清样本，通过ELISA检测均为阴性。该检测方法已常规使用多年，证明具有特异性、敏感性，适用于大量血清样本的筛查。
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