Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China.
Technical University of Liberec, Liberec, Czechia.
Carbohydr Polym. 2017 Jun 15;166:329-337. doi: 10.1016/j.carbpol.2017.02.093. Epub 2017 Feb 24.
Vibrionaceae is bifolded drug resistant emerging pathogens, active in various aquaculture sectors especially in shrimp culture around the world. V. parahaemolyticus are most dreadful viral outbreaks to shrimp culture. During the last decade the shrimp production level has been increased and also accompanied with various diseases. In the present research bacterial cellulose (BC) membrane produced by Gluconacetobacter xylinus in cell suspension. Further, TEMPO (2,2,6,6-tetramethylpiperidine-1-oxyl radical) oxidation was done to activate carboxyl group subsequently, TEMPO oxidized BC immersed in AgNO solution to generate AgNP anchored with BC to increase vibriocidal activity. Investigation of pure BC, TEMPO-BC and AgNP deposited BC was done by scanning electron microscopy (SEM), energy-dispersive spectroscopy (EDS) and X-ray diffraction (XRD). Further vibriocidal effect was observed against the pathogen V. parahaemolyticus and V. harveyi. We found AgNP-BC more effective as compare pure BC, TEMP-BC, and AgNO Therefore AgNP deposited BC, is promising alternative to control the shrimp pathogen.
弧菌科是一种折叠式耐药新兴病原体，活跃于各种水产养殖领域，特别是在全球的虾类养殖中。副溶血弧菌是对虾类养殖最可怕的病毒爆发。在过去的十年中，虾类产量有所增加，同时也伴随着各种疾病。在本研究中，木醋杆菌在细胞悬浮液中产生细菌纤维素（BC）膜。进一步，用 TEMPO（2,2,6,6-四甲基哌啶-1-氧自由基）氧化来激活羧基，随后将 TEMPO 氧化的 BC 浸入 AgNO 溶液中，生成与 BC 结合的 AgNP，以提高杀菌活性。通过扫描电子显微镜（SEM）、能谱（EDS）和 X 射线衍射（XRD）对纯 BC、TEMPO-BC 和 AgNP 沉积 BC 进行了研究。进一步观察了对病原体副溶血弧菌和溶藻弧菌的杀菌效果。我们发现 AgNP-BC 比纯 BC、TEMPO-BC 和 AgNO 更有效。因此，AgNP 沉积的 BC 是控制虾类病原体的有前途的替代物。
