DRDO BU CLS, Bharathiar University Campus, Coimbatore, 641046, India.
Department of Biochemistry and Biotechnology, CSIR-Central Leather Research Institute, Chennai, 600020, India.
Carbohydr Polym. 2020 Oct 1;245:116573. doi: 10.1016/j.carbpol.2020.116573. Epub 2020 Jun 10.
In the present study, bacterial cellulose (BC) based nanocomposite dressing material was developed for third burn wound management by polydopamine (PD) coated BC with in situ reduction of silver nanoparticles (BC-PDAg). BC-PDAg nanocomposite was characterized to understand the morphological, physical and chemical properties. Antimicrobial activity of BC-PDAg against burn wound specific pathogens were significant. The in vitro cytotoxicity and proliferation studies revealed that BC-PDAg nanocomposite is biocompatible and it supports cell proliferation. Further, in vivo experiments on female albino Wistar rats confirmed that BC-PDAg was effective in wound healing by promoting re-epithelization, and collagen deposition as evidenced by histopathological analysis. Moreover, molecular gene expression study has revealed that BC-PDAg promotes healing process by regulating the expression of inflammatory, angiogenesis and growth factor genes. The overall performance of BC-PDAg nanocomposite suggests that it could be used as promising skin regenerative tool in modern medicine.
在本研究中，通过聚多巴胺 (PD) 涂层的细菌纤维素 (BC) 原位还原纳米银颗粒，开发了用于治疗三度烧伤的基于细菌纤维素的纳米复合敷料材料 (BC-PDAg)。对 BC-PDAg 纳米复合材料进行了表征，以了解其形态、物理和化学性质。BC-PDAg 对烧伤特定病原体的抗菌活性非常显著。体外细胞毒性和增殖研究表明，BC-PDAg 纳米复合材料具有生物相容性，支持细胞增殖。此外，在雌性白化 Wistar 大鼠的体内实验证实，BC-PDAg 通过促进再上皮化和胶原沉积，在组织病理学分析中证实了其在伤口愈合方面的有效性。此外，分子基因表达研究表明，BC-PDAg 通过调节炎症、血管生成和生长因子基因的表达来促进愈合过程。BC-PDAg 纳米复合材料的整体性能表明，它可能成为现代医学中一种有前途的皮肤再生工具。
