Bishop David P, Blanes Lucas, Wilson Alexander B, Wilbanks Thor, Killeen Kevin, Grimm Rudolf, Wenzel Ross, Major Derek, Macka Mirek, Clarke David, Schmid Robin, Cole Nerida, Doble Philip A
Elemental Bio-imaging Facility, University of Technology Sydney, Broadway, New South Wales, 2007, Australia.
Agilent Technologies, Santa Clara, CA, USA.
J Chromatogr A. 2017 May 12;1497:64-69. doi: 10.1016/j.chroma.2017.03.025. Epub 2017 Mar 19.
The Agilent Chip Cube Interface is a microfluidic chip-based technology originally designed for nanospray molecular mass spectrometry in which the sample enrichment, nano-column, tubing, connectors and spray tip were integrated into a single biocompatible chip. Here we describe the hyphenation of the Chip Cube Interface to ICP-MS via modification of the standard HPLC chip design and a new total consumption nebuliser suitable for flow rates as low as 300nLmin. The potential of the instrument to eliminate common nanoLC - ICP-MS shortcomings such as leaks, blockages and band-broadening was demonstrated via analysis of cyanocobalamin in equine plasma. The method was linear over three orders of magnitude with an r of 0.9999, the peak area repeatability was 1.9% (n=7), and the detection limit was 14ngmL. This novel configuration of the Chip Cube Interface coupled to ICP-MS is a suitable platform for the analysis of biomolecules associated with trace metals and speciation applications.
安捷伦芯片立方体接口是一种基于微流控芯片的技术，最初设计用于纳米喷雾质谱分析，其中样品富集、纳柱、管路、连接器和喷雾尖端都集成在一个单一的生物相容性芯片中。在这里，我们描述了通过修改标准HPLC芯片设计以及一种适用于低至300nL/min流速的新型全消耗雾化器，将芯片立方体接口与ICP-MS联用。通过对马血浆中钴胺素的分析，证明了该仪器消除常见的纳升液相色谱-电感耦合等离子体质谱缺点（如泄漏、堵塞和谱带展宽）的潜力。该方法在三个数量级上呈线性，r为0.9999，峰面积重复性为1.9%（n=7），检测限为14ng/mL。这种与ICP-MS联用的芯片立方体接口的新型配置是分析与痕量金属相关的生物分子和形态分析应用的合适平台。
