Wang Anqi, Sun Lipei, Wang Mingshu, Jia Renyong, Zhu Dekang, Liu Mafeng, Sun Kunfeng, Yang Qiao, Wu Ying, Chen Xiaoyue, Cheng Anchun, Chen Shun
Institute of Preventive Veterinary Medicine, Sichuan Agricultural University, Chengdu, Sichuan 611130, China.
Institute of Preventive Veterinary Medicine, Sichuan Agricultural University, Chengdu, Sichuan 611130, China; Avian Disease Research Center, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, Sichuan 611130, China; Key Laboratory of Animal Disease and Human Health of Sichuan Province, Sichuan Agricultural University, Chengdu, Sichuan 611130, China.
Biomed Res Int. 2017;2017:5149062. doi: 10.1155/2017/5149062. Epub 2017 Mar 13.
As interferon-stimulated genes (ISGs), interferon-inducible transmembrane proteins 1 and 3 (IFITM1 and IFITM3) can effectively inhibit the replication of multiple viruses. Here, goose IFITM1 and IFITM3 were cloned and identified for the first time. The two proteins share the same topological structure and several important sites critical for the antiviral functions in other species are conserved in the goose. Goose IFITM1 and IFITM3 are most closely related to their respective orthologs in ducks; these proteins exhibited high mRNA transcript levels in immune-related tissues, including the thymus, bursa of Fabricius, and Harderian gland, compared to other tissues. Moreover, goose IFITM1 was highly constitutively expressed in gastrointestinal tract tissues, while goose IFITM3 was expressed in respiratory organs. Furthermore, goose IFITM3 was activated in goose peripheral blood mononuclear cells (PBMCs) infected with Tembusu virus (TMUV) or treated with Toll-like receptors (TLRs) agonists, while only the R848 and Poly (I:C) agonists induced significant upregulation of goose IFITM1. Furthermore, goose IFITM1 and IFITM3 were upregulated in the sampled tissues, to some extent, after TMUV infection. Notably, significant upregulation of goose IFITM1 and IFITM3 was detected in the cecum and cecal tonsil, where TMUV was primarily distributed. These data provide new insights into the immune effectors in geese and promote our understanding of the role of IFITM1 and IFITM3 in the defense against TMUV.
作为干扰素刺激基因（ISG），干扰素诱导跨膜蛋白1和3（IFITM1和IFITM3）可有效抑制多种病毒的复制。在此，首次克隆并鉴定了鹅IFITM1和IFITM3。这两种蛋白具有相同的拓扑结构，且在其他物种中对抗病毒功能至关重要的几个重要位点在鹅中保守。鹅IFITM1和IFITM3与其在鸭中的各自直系同源物关系最为密切；与其他组织相比，这些蛋白在包括胸腺、法氏囊和哈德氏腺在内的免疫相关组织中表现出高mRNA转录水平。此外，鹅IFITM1在胃肠道组织中高度组成性表达，而鹅IFITM3在呼吸器官中表达。此外，鹅IFITM3在感染坦布苏病毒（TMUV）或用Toll样受体（TLR）激动剂处理的鹅外周血单个核细胞（PBMC）中被激活，而只有R848和聚肌苷酸-聚胞苷酸（Poly（I:C））激动剂诱导鹅IFITM1显著上调。此外，TMUV感染后，鹅IFITM1和IFITM3在采样组织中在一定程度上被上调。值得注意的是，在TMUV主要分布的盲肠和盲肠扁桃体中检测到鹅IFITM1和IFITM3显著上调。这些数据为鹅的免疫效应器提供了新的见解，并促进了我们对IFITM1和IFITM3在抵御TMUV中的作用的理解。
