Chotikasemsri Pongsathorn, Tangtrakulwanich Boonsin, Sangkhathat Surasak
Biomedical Engineering Institute, Faculty of Medicine, Prince of Songkla University, Hat Yai, Songkhla, Thailand.
Department of Orthopedic Surgery and Physical Medicine, Faculty of Medicine, Prince of Songkla University, Hat Yai, Songkhla, Thailand.
Biomed Res Int. 2017;2017:7604861. doi: 10.1155/2017/7604861. Epub 2017 Mar 12.
The purpose of this study was to investigate whether LED light at different wavelengths affects the expression profile of 143 cancer predisposition genes in both diabetic and normal human fibroblasts. In this study, both diabetic and normal fibroblast cell lines were cultured and irradiated with red (635 nm), green (520 nm), and blue (465 nm) LED light for 10 minutes at 0.67 J/cm each. After that, mRNA from all cell lines was extracted for microarray analysis. We found that green light activates EPHB2, KIT, ANTXR2, ESCO2, MSR1, EXT1, TSC1, KIT, NF1, BUB1B, FANCD2, EPCAM, FANCD2, NF, DIS3L2, and RET in normal fibroblast cells, while blue and red light can upregulate RUNX1, PDGFRA, EHBP1, GPC3, AXIN2, KDR, GLMN, MSMB, EPHB2, MSR1, KIT, FANCD2, BMPR1A, BUB1B, PDE11A, and RET. Therefore, genetic screening before phototherapy treatment may be required.
本研究的目的是调查不同波长的LED光是否会影响糖尿病患者和正常人成纤维细胞中143种癌症易感基因的表达谱。在本研究中，培养糖尿病和成纤维细胞系，并用红色（635nm）、绿色（520nm）和蓝色（465nm）LED光以0.67J/cm²的强度照射10分钟。之后，提取所有细胞系的mRNA进行微阵列分析。我们发现，绿光可激活正常成纤维细胞中的EPHB2、KIT、ANTXR2、ESCO2、MSR1、EXT1、TSC1、KIT、NF1、BUB1B、FANCD2、EPCAM、FANCD2、NF、DIS3L2和RET，而蓝光和红光可上调RUNX1、PDGFRA、EHBP1、GPC3、AXIN2、KDR、GLMN、MSMB、EPHB2、MSR1、KIT、FANCD2、BMPR1A、BUB1B、PDE11A和RET。因此，光疗前可能需要进行基因筛查。
