Ribeiro Lucas R, Santos Ana R O, Groenewald Marizeth, Smith Maudy Th H, Lara Carla A, Góes-Neto Aristóteles, Jacques Noémie, Grondin Cécile, Casaregola Serge, Lachance Marc-André, Rosa Carlos A
Departamento de Microbiologia, ICB, C.P. 486, Universidade Federal de Minas Gerais, Belo Horizonte, MG, 31270-901, Brazil.
Westerdijk Fungal Biodiversity Institute, Utrecht, The Netherlands.
Antonie Van Leeuwenhoek. 2017 Jul;110(7):985-994. doi: 10.1007/s10482-017-0870-2. Epub 2017 Apr 6.
During studies of the yeast diversity associated with rotting wood in Brazil and fruits, plants and insects in French Guiana, three strains of a new species were isolated. Analysis of the sequences of the internal transcribed spacer (ITS)-5.8S and D1/D2 domains of the large subunit of the rRNA gene showed that this species belongs to the genus Hyphopichia and its closest relative is Candida homilentoma. These species differ by 44 nucleotide substitutions in D1/D2 sequences. A new species Hyphopichia buzzinii f. a., sp. nov., is proposed to accommodate these isolates. The type strain of Hyphopichia buzzinii sp. nov. is CLIB 1739 (=CBS 14300 = UFMG-CM-Y6121; MycoBank number is MB 815609). In addition, we isolated 11 strains of C. homilentoma from rotting wood, leaf surfaces, and water bodies in Brazil, and these strains when crossed among one another and with the type strain (CBS 6312) of this species, produced hat-shaped ascospores typical of the genus Hyphopichia. We describe the teleomorph of C. homilentoma as a new combination, Hyphopichia homilentoma comb. nov. (type strain CBS 6312; MycoBank number is MB 820009). We also propose to transfer the other six Candida species of the Hyphopichia clade to this genus as new combinations. Hyphopichia homilentoma produced ethanol and xylitol from D-xylose whereas H. buzzinii was only able to convert this pentose to xylitol.
在对巴西腐烂木材以及法属圭亚那的水果、植物和昆虫相关的酵母多样性进行研究期间，分离出了三株新物种的菌株。对核糖体RNA基因大亚基的内部转录间隔区（ITS）-5.8S以及D1/D2结构域的序列分析表明，该物种属于丝孢酵母属，其最相近的亲缘种是霍氏假丝酵母。这些物种在D1/D2序列中有44个核苷酸替换。现提出一个新物种——布氏丝孢酵母新变种（Hyphopichia buzzinii f. a., sp. nov.）来容纳这些分离株。布氏丝孢酵母新种（Hyphopichia buzzinii sp. nov.）的模式菌株为CLIB 1739（=CBS 14300 = UFMG-CM-Y6121；国际真菌名称数据库编号为MB 815609）。此外，我们从巴西的腐烂木材、叶片表面和水体中分离出了11株霍氏假丝酵母，这些菌株相互之间以及与该物种的模式菌株（CBS 6312）杂交时，产生了丝孢酵母属典型的帽形子囊孢子。我们将霍氏假丝酵母的有性型描述为一个新组合——霍氏丝孢酵母（Hyphopichia homilentoma comb. nov.）（模式菌株CBS 6312；国际真菌名称数据库编号为MB 820009）。我们还提议将丝孢酵母分支中的其他六个假丝酵母物种作为新组合转移到该属。霍氏丝孢酵母能利用D-木糖产生乙醇和木糖醇，而布氏丝孢酵母只能将这种戊糖转化为木糖醇。
