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sp. nov.,一种从腐朽木材和天牛幼虫粪便中分离出的产木糖醇酵母物种。

sp. nov., a xylitol-producing yeast species isolated from rotting wood and frass of cerambycid larva.

机构信息

Departamento de Microbiologia, ICB, C.P. 486, Universidade Federal de Minas Gerais, Belo Horizonte, MG, 31270-901, Brazil.

Department of Biology, City College and the Graduate Center, The City University of New York, Convent Avenue at 138 St., New York, NY 10031, USA.

出版信息

Int J Syst Evol Microbiol. 2021 Sep;71(9). doi: 10.1099/ijsem.0.004986.

DOI:10.1099/ijsem.0.004986
PMID:34494946
Abstract

Six yeast isolates were obtained from rotting wood samples in Brazil and frass of a cerambycid beetle larva in French Guiana. Sequence analysis of the ITS-5.8S region and the D1/D2 domains of the large subunit rRNA gene showed that the isolates represent a novel species of . This novel species is related to , , and . It is heterothallic and produces asci with two or four hat-shaped ascospores. The name sp. nov. is proposed to accommodate the novel species. The holotype of is CBS 16129 and the designated paratype is CBS 16584. The MycoBank number is 838252. All isolates of were able to convert xylose into xylitol with maximum xylitol production within 60 and 72 h. The isolates produced xylitol with values ranging from 12.61 to 31.79 g l in yeast extract-peptone-xylose medium with 5% xylose. When the isolates were tested in sugarcane bagasse hydrolysate containing around 35-38 g ld-xylose, isolate UFMG-CM-Y519 showed maximum xylitol production.

摘要

从巴西腐烂木材样本和法属圭亚那一种天牛幼虫粪便中分离到 6 株酵母菌株。ITS-5.8S 区和大亚基 rRNA 基因的 D1/D2 区序列分析表明,这些分离株代表了一个 的新种。该新种与 、 、 和 有关。它是异宗配合的,产生具有两个或四个帽状子囊孢子的子囊。建议采用 sp. nov. 这个名称来容纳这个新种。 的模式标本是 CBS 16129,指定的副模式标本是 CBS 16584。其 MycoBank 编号为 838252。所有 的分离株都能够将木糖转化为木糖醇,在 60 和 72 小时内达到最大木糖醇产量。这些分离株在含 5%木糖的酵母提取物-蛋白胨-木糖培养基中产生的木糖醇值范围为 12.61 至 31.79 g/L。当这些分离株在含有约 35-38 g/L d-木糖的甘蔗渣水解物中进行测试时,UFMG-CM-Y519 分离株表现出最大的木糖醇产量。

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