鸡抗生物素蛋白三个结构相关基因的分子克隆

Molecular cloning of three structurally related genes for chicken avidin.

作者信息

Keinänen R A, Laukkanen M L, Kulomaa M S

机构信息

Department of Biomedical Sciences, University of Tampere, Finland.

出版信息

J Steroid Biochem. 1988;30(1-6):17-21. doi: 10.1016/0022-4731(88)90071-4.

DOI:10.1016/0022-4731(88)90071-4

PMID:2838690

Abstract

The chicken genomic library was screened using the 32P-labelled 3'-end of avidin cDNA as a hybridization probe. A positive clone, lambda gAV12201, containing a 15-16 kb insert, was detected. The EcoRI subclones, pgAV0.4, pgAV1.8, pgAV3.3 and pgAV3.7 from the genomic clone were subjected to hybridization and restriction enzyme mapping analysis. The preliminary results suggest the existence of three structurally related genes for chicken avidin. Whether the natural gene is within the subclones can only be established when sequencing analyses of the subclones have been completed.

摘要

使用生物素 cDNA 的 32P 标记 3' 末端作为杂交探针筛选鸡基因组文库。检测到一个阳性克隆 λgAV12201，其包含一个 15 - 16 kb 的插入片段。对来自基因组克隆的 EcoRI 亚克隆 pgAV0.4、pgAV1.8、pgAV3.3 和 pgAV3.7 进行杂交和限制性酶切图谱分析。初步结果表明鸡生物素存在三个结构相关的基因。只有当亚克隆的测序分析完成后，才能确定天然基因是否在这些亚克隆中。