Delarue C, Lihrmann I, Feuilloley M, Netchitaïlo P, Idres S, Leboulenger F, Bélanger A, Perroteau I, Vaudry H
Groupe de Recherche en Endocrinologie Moléculaire, UA CNRS 650, Université de Rouen, Mont-Saint-Aignan, France.
J Steroid Biochem. 1988;30(1-6):461-4. doi: 10.1016/0022-4731(88)90143-4.
The possible role of arachidonic acid metabolites of the lipoxygenase pathway in the regulation of steroidogenesis was studied in vitro using perifused frog interrenal (adrenal) glands. Graded doses of arachidonic acid (10(-6)-10(-4)M) increased the production of corticosterone and aldosterone in a dose-dependent manner. In the presence of indomethacin (5 X 10(-6)M), the effect of arachidonic acid on steroid secretion was totally abolished. Nordihydroguaiaretic acid (NDGA: 10(-6)M), a lipoxygenase inhibitor, did not alter the spontaneous secretion of corticosteroids and did not impair the stimulatory effect of arachidonic acid. In the presence of NDGA, both ACTH and angiotensin II were still able to stimulate corticosteroid production. Our data support the view that arachidonic acid metabolites play an important role in the regulation of amphibian steroidogenesis. Moreover, the results show that the lipoxygenase pathway is not involved in the spontaneous secretion of corticosteroids and in angiotensin II- or ACTH-induced steroidogenesis.
利用灌流蛙肾间（肾上腺）腺体外培养法，研究了脂氧合酶途径中花生四烯酸代谢产物在类固醇生成调节中的可能作用。不同剂量的花生四烯酸（10^(-6)-10^(-4)M）以剂量依赖方式增加了皮质酮和醛固酮的生成。在吲哚美辛（5×10^(-6)M）存在的情况下，花生四烯酸对类固醇分泌的作用完全被消除。去甲二氢愈创木酸（NDGA：10^(-6)M），一种脂氧合酶抑制剂，既不改变皮质类固醇的自发分泌，也不削弱花生四烯酸的刺激作用。在NDGA存在的情况下，促肾上腺皮质激素（ACTH）和血管紧张素II仍能刺激皮质类固醇的生成。我们的数据支持这样一种观点，即花生四烯酸代谢产物在两栖类动物类固醇生成的调节中起重要作用。此外，结果表明脂氧合酶途径不参与皮质类固醇的自发分泌以及血管紧张素II或ACTH诱导的类固醇生成。
