Draetta G, Beach D, Moran E
Cold Spring Harbor Laboratory, New York 11724.
Oncogene. 1988 Jun;2(6):553-7.
The homolog of the cdc2+/CDC28+ encoded protein kinases has previously been identified in HeLa cells by immunological methods. Here we have studied the distribution and synthesis of this 34 kd protein in rat tissues and cell lines. p34 was found in a variety of organs, including some such as brain that are not highly active in cell division. Kidney has very low levels of p34. However, proliferative activation of baby rat kidney cells with adenovirus caused rapid induction of p34 synthesis. Induction was dependent on the E1A gene of the virus but not E1B, and was not prevented by inhibition of cellular DNA synthesis with hydroxyurea. Increased synthesis of p34 is due, at least in part, to an increase in abundance of translatable p34 mRNA. These data are consistent with the possibility that p34 plays a role in cell division in higher vertebrates.
通过免疫学方法，先前已在HeLa细胞中鉴定出与cdc2+/CDC28+编码的蛋白激酶同源的蛋白。在此，我们研究了这种34kd蛋白在大鼠组织和细胞系中的分布及合成情况。在多种器官中都发现了p34，包括一些如大脑等细胞分裂不活跃的器官。肾脏中p34的水平非常低。然而，用腺病毒对新生大鼠肾细胞进行增殖激活会导致p34合成的快速诱导。诱导作用依赖于病毒的E1A基因而非E1B基因，并且用羟基脲抑制细胞DNA合成并不能阻止这种诱导。p34合成的增加至少部分是由于可翻译的p34 mRNA丰度的增加。这些数据与p34在高等脊椎动物细胞分裂中发挥作用的可能性相一致。
