Alonso Cristina, Martí M, Barba C, Carrer V, Rubio L, Coderch L
Department of Chemicals and Surfactants Technology, Advanced Chemical Institute of Catalonia (IQAC-CSIC), Jordi Girona 18-26, 08034, Barcelona, Spain.
Arch Dermatol Res. 2017 Aug;309(6):423-431. doi: 10.1007/s00403-017-1740-5. Epub 2017 Apr 7.
The permeation of resveratrol was assessed by in vitro and in vivo experiments 24 h after topical administration. The in vitro profile of resveratrol was assessed by Raman spectroscopy. Human skin permeation was analysed in vivo by the tape stripping method with the progressive removal of the stratum corneum layers using adhesive tape strips. Moreover, the free radical scavenging activity of resveratrol after its topical application was determined using the DPPH assay. The Raman spectra indicated that the topically applied resveratrol penetrates deep into the skin. The results showed high amounts of resveratrol in the different stratum corneum layers close to the surface and a constant lower amount in the upper layers of the viable epidermis. The concentration of resveratrol present in the outermost stratum corneum layers was obtained by tape stripping after in vivo application. The results demonstrated that resveratrol mainly remained in the human stratum corneum layers. After topical application, resveratrol maintained its antiradical activity. The antioxidant efficacy of the compound was higher in the inner layers of the stratum corneum. As these results have demonstrated, topically applied resveratrol reinforces the antioxidant system of the stratum corneum and provides an efficient means of increasing the tissue levels of antioxidants in the human epidermis.
在局部给药24小时后，通过体外和体内实验评估白藜芦醇的渗透情况。通过拉曼光谱法评估白藜芦醇的体外特性。通过胶带剥离法在体内分析人体皮肤渗透情况，使用胶带条逐步去除角质层。此外，使用DPPH测定法测定局部应用后白藜芦醇的自由基清除活性。拉曼光谱表明，局部应用的白藜芦醇可深入皮肤。结果显示，靠近表面的不同角质层中有大量白藜芦醇，而在活表皮上层中含量较低且恒定。在体内应用后，通过胶带剥离获得最外层角质层中白藜芦醇的浓度。结果表明，白藜芦醇主要保留在人体角质层中。局部应用后，白藜芦醇保持其抗自由基活性。该化合物的抗氧化功效在角质层内层更高。正如这些结果所示，局部应用白藜芦醇可增强角质层的抗氧化系统，并提供一种提高人体表皮组织抗氧化剂水平的有效方法。
