Youssef Mohamed Ragab, Attia Zeinab Ibraheim, El-Baz Rizk Ahmed, Roshdy Sameh, Settin Ahmad
Physiology Department, Faculty of Science, Tanta University, Tanta, Egypt.
Zoology Department, Faculty of Science, Tanta University, Tanta, Egypt.
Fam Cancer. 2017 Oct;16(4):517-524. doi: 10.1007/s10689-017-9992-y.
To assess the association of genetic polymorphisms of NFκB1 and NFκBIA genes with the susceptibility to colorectal cancer (CRC). Subjects included 100 Egyptian patients with CRC (60 males and 40 females) in addition to 85 healthy controls (47 males and 38 females) from the same locality. For all participants, genetic polymorphisms of NFκB1-94ins/delATTG (rs28362491) and NFκBIA-881A/G (rs3138053) were detected by using restriction fragment length polymorphism polymerase chain reaction (RFLP-PCR). CRC patients showed a significantly higher frequency of the NFκB1-94ins/ins genotype than controls (30 vs. 4.7%) that was significant in the recessive (OR 17.69, 95% CI 5.41-57.82, p < 0.0001) and codominant models (OR 18.28, 95% CI 4.87-68.6, p < 0.0001). The NFκB1-94ins allele frequency was significantly higher among patients than controls (58 vs. 39%, OR 2.18, 95% CI 1.4-3.3, p = 0.0004). We also noticed that the genotype G/G of NFκBIA-881 polymorphism was present in patients (4%) while it was absent (0%) in controls with increased frequency of the NFκBIA-881G allele in patients compared to controls (23 vs. 14%, p = 0.041). These polymorphisms were more associated with smoking and advanced tumor staging. This study indicates that the NFκB1-94ins/ins genotype was associated with the risk of developing colorectal cancer in Egyptian subjects. Also, CRC cases showed an increase in the frequency of NFκBIA-881G allele but not reaching statistical significance for multiple comparisons.
评估NFκB1和NFκBIA基因的遗传多态性与结直肠癌（CRC）易感性之间的关联。研究对象包括100名埃及CRC患者（60名男性和40名女性）以及来自同一地区的85名健康对照者（47名男性和38名女性）。对所有参与者，采用限制性片段长度多态性聚合酶链反应（RFLP-PCR）检测NFκB1-94ins/delATTG（rs28362491）和NFκBIA-881A/G（rs3138053）的遗传多态性。CRC患者中NFκB1-94ins/ins基因型的频率显著高于对照组（30%对4.7%），在隐性模型（OR 17.69，95%CI 5.41-57.82，p<0.0001）和共显性模型（OR 18.28，95%CI 4.87-68.6，p<0.0001）中均具有显著性。患者中NFκB1-94ins等位基因频率显著高于对照组（58%对39%，OR 2.18，95%CI 1.4-3.3，p=0.0004）。我们还注意到，NFκBIA-881多态性的G/G基因型在患者中存在（4%），而在对照组中不存在（0%），患者中NFκBIA-881G等位基因频率高于对照组（23%对14%，p=0.041）。这些多态性与吸烟和肿瘤晚期分期更相关。本研究表明，NFκB1-94ins/ins基因型与埃及受试者患结直肠癌的风险相关。此外，CRC病例中NFκBIA-881G等位基因频率有所增加，但在多重比较中未达到统计学显著性。
