马萨利亚磁球菌MO-1菌株自由游动运动性和趋磁行为的测量
Measurement of Free-Swimming Motility and Magnetotactic Behavior of Magnetococcus massalia Strain MO-1.
作者信息
Zhang Wei-Jia, Zhang Sheng-Da, Wu Long-Fei
机构信息
Laboratory of Deep-Sea Microbial Cell Biology, Sanya Institute of Deep-Sea Science and Engineering, Chinese Academy of Sciences, Sanya, China.
Laboratoire International Associé de la Bio-Minéralisation et Nano-Structures (LIA-BioMNSL), Centre National de la Recherche Scientifique, F-13402, Marseille cedex 20, France.
出版信息
Methods Mol Biol. 2017;1593:305-320. doi: 10.1007/978-1-4939-6927-2_25.
Magnetococcus massalia strain MO-1 represents a group of fast-swimming marine magnetotactic coccoid-ovoid bacteria. They show polar magnetotaxis behavior in uniform magnetic field. MO-1 cells swim forward constantly with rare stop. When they meet obstacles, MO-1 cells could squeeze through or circumvent the obstacles. Here, we describe the methods for characterization of magnetotactic behaviors of MO-1 cells using adapted spectrophotometer and microscope mounted with magnetic fields.
马赛磁球菌MO-1菌株代表了一类快速游动的海洋趋磁球状-卵形细菌。它们在均匀磁场中表现出极性趋磁行为。MO-1细胞持续向前游动，很少停止。当遇到障碍物时，MO-1细胞可以挤过或绕过障碍物。在这里，我们描述了使用配备磁场的适配分光光度计和显微镜来表征MO-1细胞趋磁行为的方法。
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