Shah Sunil, Chandra Anjali, Kaur Amanjot, Sabnis Nirupama, Lacko Andras, Gryczynski Zygmunt, Fudala Rafal, Gryczynski Ignacy
Institute for Molecular Medicine, Program in Fluorescence Technologies at Center for Cancer Research, University of North Texas Health Science Center, Fort Worth, TX 76107, USA.
Institute for Molecular Medicine, Program in Fluorescence Technologies at Center for Cancer Research, University of North Texas Health Science Center, Fort Worth, TX 76107, USA.
J Photochem Photobiol B. 2017 May;170:65-69. doi: 10.1016/j.jphotobiol.2017.03.024. Epub 2017 Mar 30.
We studied steady-state and time-resolved fluorescence properties of an anticancer drug Doxorubicin in a saline buffer and poly-vinyl alcohol (PVA) film. Absorption of Doxorubicin, located at blue-green spectral region, allows a convenient excitation with visible light emitting diodes or laser diodes. Emission of Doxorubicin with maximum near 600nm can be easily detected with photomultipliers and CCD cameras. Both, absorption and fluorescence spectra in polymeric matrix show more pronounced vibronic structures than in solution. Also, the steady-state anisotropy in the polymer film is significantly higher than in the saline solution. In PVA film the fluorescence anisotropy is about 0.30 whereas in the saline buffer only 0.07. Quantum efficiencies of Doxorubicin were compared to a known standard Rhodamine 101 which has fluorescence emission in a similar spectral region. The quantum yield of Doxorubicin in PVA film is more than 10% and about twice higher than in the saline solution. Similarly, the lifetime of doxorubicin in PVA film is about 2ns whereas in the saline solution only about 1ns. The fluorescence anisotropy decays show that Doxorubicin molecules are freely rotating in the saline buffer with a correlation time of about 290ps, and are almost completely immobilized in the PVA film. The spectroscopic investigations presented in this manuscript are important, as they provide answers to changes in molecular properties of Doxorubicin depending changes in the local environment, which is useful when synthesizing nanoparticles for Doxorubicin entrapment.
我们研究了抗癌药物阿霉素在盐缓冲液和聚乙烯醇（PVA）薄膜中的稳态和时间分辨荧光特性。阿霉素的吸收位于蓝绿色光谱区域，便于使用可见光发光二极管或激光二极管进行激发。阿霉素在600nm附近具有最大发射峰，其发射光可用光电倍增管和电荷耦合器件相机轻松检测到。与在溶液中相比，聚合物基质中的吸收光谱和荧光光谱都显示出更明显的振动结构。此外，聚合物薄膜中的稳态各向异性明显高于盐溶液中的。在PVA薄膜中，荧光各向异性约为0.30，而在盐缓冲液中仅为0.07。将阿霉素的量子效率与已知标准罗丹明101进行了比较，罗丹明101在相似光谱区域有荧光发射。阿霉素在PVA薄膜中的量子产率超过10%，约为盐溶液中的两倍。同样，阿霉素在PVA薄膜中的寿命约为2ns，而在盐溶液中仅约为1ns。荧光各向异性衰减表明，阿霉素分子在盐缓冲液中自由旋转，相关时间约为290ps，而在PVA薄膜中几乎完全固定。本手稿中提出的光谱研究很重要，因为它们为阿霉素分子性质随局部环境变化提供了答案，这在合成用于包裹阿霉素的纳米颗粒时很有用。
