Chowdhury Abhra Chandra, Chaurasia Smriti, Mishra Shravan Kumar, Aggarwal Amita, Misra Ramnath
Department of Clinical Immunology, Sanjay Gandhi Postgraduate Institute of Medical Sciences, Lucknow Pin: 226014, India.
Department of Clinical Immunology, Sanjay Gandhi Postgraduate Institute of Medical Sciences, Lucknow Pin: 226014, India.
Clin Immunol. 2017 Oct;183:207-212. doi: 10.1016/j.clim.2017.03.016. Epub 2017 Apr 6.
The IL-17/1L-23 axis is important in the pathogenesis of spondyloarthropathy. Innate cells produce IL-17 in addition to Th17 cells. We studied the frequencies of natural killer (NK) (total, CD56, CD56, perforin+ and granzyme+), NK-T, γδ-T, and IFN-γ+, IL-17+ NK and γδ-T cells in peripheral blood (PB) and synovial fluid (SF) of ReA/uSpA patients. PB from 45 patients and paired SF from 39 patients were studied, together with PB from 18 healthy controls (HC). The frequency of γδ-T cells was decreased (p<0.05) while IL-17 producing NK and γδ-T cells were increased (p<0.05) in PB of patients as compared to HC. In SF, CD56 NK cells were increased (p<0.001) but had reduced expression of perforin and granzyme (p<0.0001) as compared to PB. Frequency of IL-17+, IFN-γ+ NK and γδ-T cells was higher in SF as compared to PB (p<0.05). We suggest that innate cells by producing pro-inflammatory cytokines may contribute to pathogenesis.
白细胞介素-17/白细胞介素-23轴在脊柱关节炎的发病机制中起重要作用。除了辅助性T细胞17（Th17）外，固有细胞也会产生白细胞介素-17。我们研究了反应性关节炎/未分化脊柱关节炎（ReA/uSpA）患者外周血（PB）和滑液（SF）中自然杀伤（NK）细胞（总数、CD56、CD56、穿孔素+和颗粒酶+）、自然杀伤T细胞（NK-T）、γδ-T细胞以及干扰素-γ+、白细胞介素-17+的NK细胞和γδ-T细胞的频率。研究了45例患者的外周血以及39例患者配对的滑液，同时纳入了18名健康对照（HC）的外周血。与健康对照相比，患者外周血中γδ-T细胞频率降低（p<0.05），而产生白细胞介素-17的NK细胞和γδ-T细胞增加（p<0.05）。在滑液中，CD56 NK细胞增加（p<0.001），但与外周血相比，穿孔素和颗粒酶的表达降低（p<0.0001）。与外周血相比，滑液中白细胞介素-17+、干扰素-γ+的NK细胞和γδ-T细胞频率更高（p<0.05）。我们认为，固有细胞通过产生促炎细胞因子可能参与了发病机制。
