Shen Xiu-Yu, Yu Yang, Chen Guo-Dong, Zhou Hua, Luo Jin-Fang, Zuo Yi-Han, Yao Xin-Sheng, Dai Yi
College of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, Shenyang 110016, People's Republic of China.
Institute of Traditional Chinese Medicine and Natural Products, College of Pharmacy, Jinan University, Guangzhou 510632, People's Republic of China.
Fitoterapia. 2017 Jun;119:75-82. doi: 10.1016/j.fitote.2017.04.004. Epub 2017 Apr 5.
Six new sesquiterpenoids, namely nardosinanones J-N and nardoaristolone C, were isolated from the rhizomes and roots of Nardostachys chinensis Batal. Their structures were determined by interpretation of spectroscopic data (HR-ESI-MS, 1D and 2D NMR). A combination of X-ray crystal diffraction, ECD calculation, and Mosher ester methods was employed to determine the absolute configuration of the isolated compounds. Compounds 1-2, 4-6 were evaluated anti-inflammatory activities in LPS-stimulated RAW264.7 macrophages. The results showed that compound 5 obviously inhibited LPS-induced iNOS and COX-2 protein expression compared to single LPS stimulation, which indicated the potential effect to medicate anti-inflammatory.
从甘青青兰的根茎和根中分离出6个新的倍半萜类化合物，即甘青青兰酮J-N和甘青青兰甾酮C。通过光谱数据（高分辨电喷雾电离质谱、一维和二维核磁共振）解析确定了它们的结构。采用X射线晶体衍射、电子圆二色计算和莫舍尔酯法相结合的方法确定了分离得到化合物的绝对构型。对化合物1-2、4-6在脂多糖刺激的RAW264.7巨噬细胞中进行抗炎活性评价。结果表明，与单独脂多糖刺激相比，化合物5明显抑制脂多糖诱导的诱导型一氧化氮合酶和环氧化酶-2蛋白表达，这表明其具有潜在的抗炎药用作用。
