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Ectoenzymes of the kidney microvillar membrane. Differential solubilization by detergents can predict a glycosyl-phosphatidylinositol membrane anchor.肾微绒毛膜的外切酶。去污剂的差异增溶作用可预测糖基磷脂酰肌醇膜锚定物。
Biochem J. 1988 Mar 15;250(3):865-9. doi: 10.1042/bj2500865.
2
Activation of the glycosyl-phosphatidylinositol-anchored membrane dipeptidase upon release from pig kidney membranes by phospholipase C.通过磷脂酶C从猪肾膜释放后糖基磷脂酰肌醇锚定膜二肽酶的激活。
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3
Ectoenzymes of the kidney microvillar membrane. Isolation and characterization of the amphipathic form of renal dipeptidase and hydrolysis of its glycosyl-phosphatidylinositol anchor by an activity in plasma.肾微绒毛膜的外切酶。肾二肽酶两亲形式的分离与特性以及血浆中一种活性对其糖基磷脂酰肌醇锚的水解作用。
Biochem J. 1989 Aug 1;261(3):811-8. doi: 10.1042/bj2610811.
4
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Biochem J. 1991 Dec 15;280 ( Pt 3)(Pt 3):745-51. doi: 10.1042/bj2800745.
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Ectoenzymes of the kidney microvillar membrane. Aminopeptidase P is anchored by a glycosyl-phosphatidylinositol moiety.肾微绒毛膜的外切酶。氨肽酶P由一个糖基磷脂酰肌醇部分锚定。
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Glycosyl-phosphatidylinositol anchored acetylcholinesterase as substrate for phosphatidylinositol-specific phospholipase C from Bacillus cereus.糖基磷脂酰肌醇锚定的乙酰胆碱酯酶作为蜡样芽孢杆菌磷脂酰肌醇特异性磷脂酶C的底物。
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Folate binding protein from kidney brush border membranes contains components characteristic of a glycoinositol phospholipid anchor.来自肾刷状缘膜的叶酸结合蛋白含有糖基磷脂酰肌醇锚定物的特征成分。
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Priority targeting of glycosyl-phosphatidylinositol-anchored proteins to the bile-canalicular (apical) plasma membrane of hepatocytes. Involvement of 'late' endosomes.糖基磷脂酰肌醇锚定蛋白优先靶向至肝细胞胆小管（顶端）质膜。“晚期”内体的参与。
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cDNA cloning and expression in Xenopus laevis oocytes of pig renal dipeptidase, a glycosyl-phosphatidylinositol-anchored ectoenzyme.猪肾二肽酶（一种糖基磷脂酰肌醇锚定的胞外酶）的cDNA克隆及其在非洲爪蟾卵母细胞中的表达
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Partial release of aminopeptidase N from larval midgut cell membranes of the silkworm, Bombyx mori, by phosphatidylinositol-specific phospholipase C.磷脂酰肌醇特异性磷脂酶C对家蚕幼虫中肠细胞膜上氨肽酶N的部分释放
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本文引用的文献

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METHOD FOR ASSAY OF INTESTINAL DISACCHARIDASES.肠道双糖酶的测定方法。
Anal Biochem. 1964 Jan;7:18-25. doi: 10.1016/0003-2697(64)90115-0.
2
Proteins of the kidney microvillar membrane. Aspartate aminopeptidase: purification by immunoadsorbent chromatography and properties of the detergent- and proteinase-solubilized forms.肾微绒毛膜蛋白。天冬氨酸氨肽酶：通过免疫吸附色谱法纯化以及去污剂和蛋白酶增溶形式的性质
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Sulfobetaine derivatives of bile acids: nondenaturing surfactants for membrane biochemistry.胆汁酸的磺基甜菜碱衍生物：用于膜生物化学的非变性表面活性剂。
Anal Biochem. 1983 Apr 1;130(1):72-82. doi: 10.1016/0003-2697(83)90651-6.
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Association of renal dipeptidase with the Triton-insoluble fraction of kidney microvilli.肾二肽酶与肾微绒毛的不溶性 Triton 组分的关联。
J Membr Biol. 1983;75(2):115-22. doi: 10.1007/BF01995631.
5
Proteins of the kidney microvillar membrane. The amphipathic forms of endopeptidase purified from pig kidneys.肾微绒毛膜蛋白。从猪肾中纯化的内肽酶的两亲形式。
Biochem J. 1983 Jun 1;211(3):743-53. doi: 10.1042/bj2110743.
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Solubilization of phospholipids by detergents. Structural and kinetic aspects.洗涤剂对磷脂的增溶作用。结构和动力学方面。
Biochim Biophys Acta. 1983 May 24;737(2):285-304. doi: 10.1016/0304-4157(83)90004-7.
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Beta-lactamase activity of purified and partially characterized human renal dipeptidase.纯化及部分特性鉴定的人肾二肽酶的β-内酰胺酶活性
J Biol Chem. 1984 Dec 10;259(23):14586-90.
8
Solubilization and purification of rat liver 5'-nucleotidase by use of a zwitterionic detergent and a monoclonal-antibody immunoadsorbent.使用两性离子去污剂和单克隆抗体免疫吸附剂对大鼠肝脏5'-核苷酸酶进行增溶和纯化。
Biochem J. 1982 Apr 1;203(1):245-51. doi: 10.1042/bj2030245.
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Phase separation of integral membrane proteins in Triton X-114 solution.整合膜蛋白在Triton X-114溶液中的相分离。
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10
Selective release of plasma-membrane enzymes from rat hepatocytes by a phosphatidylinositol-specific phospholipase C.磷脂酰肌醇特异性磷脂酶C对大鼠肝细胞血浆膜酶的选择性释放
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肾微绒毛膜的外切酶。去污剂的差异增溶作用可预测糖基磷脂酰肌醇膜锚定物。

Ectoenzymes of the kidney microvillar membrane. Differential solubilization by detergents can predict a glycosyl-phosphatidylinositol membrane anchor.

作者信息

Hooper N M, Turner A J

机构信息

Department of Biochemistry, University of Leeds, U.K.

出版信息

Biochem J. 1988 Mar 15;250(3):865-9. doi: 10.1042/bj2500865.

DOI:10.1042/bj2500865
PMID:2839148
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1148935/
Abstract

The pattern of solubilization of nine kidney microvillar ectoenzymes by a range of detergents distinguished two classes of membrane proteins: those released from the membrane by bacterial phosphatidylinositol-specific phospholipase C and those not so released. The latter group of transmembrane proteins were solubilized efficiently (greater than 80%) by all the detergents examined. In contrast, proteins released by phosphatidylinositol-specific phospholipase C were solubilized effectively only by octyl glucoside, 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulphonate and sodium deoxycholate. Octyl glucoside solubilized the amphipathic forms of the ectoenzymes examined, suggesting that this may be a useful detergent in the purification of glycosyl-phosphatidylinositol-anchored ectoenzymes.

摘要

一系列去污剂对九种肾微绒毛外切酶的增溶模式区分出两类膜蛋白

一类是通过细菌磷脂酰肌醇特异性磷脂酶C从膜上释放出来的，另一类则不是。后一组跨膜蛋白能被所有检测的去污剂有效增溶（大于80%）。相比之下，由磷脂酰肌醇特异性磷脂酶C释放的蛋白仅能被辛基葡糖苷、3-[(3-胆酰胺丙基)二甲基铵]-1-丙烷磺酸盐和脱氧胆酸钠有效增溶。辛基葡糖苷增溶了所检测外切酶的两亲形式，这表明它可能是纯化糖基磷脂酰肌醇锚定外切酶的一种有用去污剂。