Xu Bin, Huang Shuran, Wang Caiying, Zhang Haitao, Fang Shengcun, Zhang Yingming
Department of Respiratory Medicine, Nanjing Chest Hospital, Nanjing, Jiangsu 210029, P.R. China.
Mol Med Rep. 2017 Jun;15(6):3674-3680. doi: 10.3892/mmr.2017.6428. Epub 2017 Apr 3.
Dihydromyricetin (DHM) is a plant flavonoid and is the primary active ingredient isolated from the medicinal herb, Ampelopsis grossedentata. DHM has been shown to possess various pharmacological activities, including anti‑inflammatory effects. However, the possible role of DHM in asthma treatment remains to be elucidated. The present study aimed to investigate its anti‑inflammatory properties in mice with symptoms of allergic asthma. The C57BL/6 mice were sensitized and challenged with ovalbumin (OVA) to induce asthma. DHM or phosphate‑buffered saline treatment was administered 1 h prior to the OVA challenge. The levels of interleukin (IL)‑4, IL‑5 and IL‑13 in the bronchoalveolar lavage (BAL) fluid were measured by enzyme‑linked immunosorbent assay (ELISA), and OVA‑specific serum IgE and IgG1 levels were also determined by ELISA. Histopathological staining was performed to evaluate the infiltration of inflammatory cells into the BAL fluid, lung tissues and goblet cell hyperplasia. DHM treatment significantly reduced the total number of inflammatory cells, including eosinophils, neutrophils, lymphocytes and macrophages, in the BAL fluid. DHM also reduced the levels of IL‑4, IL‑5 and IL‑13 in the BAL fluid, and reduced the secretion of OVA‑specific IgE and IgG1 in the serum. The histological staining demonstrated that DHM treatment effectively suppressed the OVA‑induced inflammatory cells in the lung tissues and in the mucus hypersecreted by goblet cells in the airway. These results showed that DHM had a potent anti‑inflammatory effect in an OVA‑induced mouse model of asthma, offering potential as an anti‑inflammatory agent for the treatment of asthma.
二氢杨梅素（DHM）是一种植物类黄酮，是从药用植物显齿蛇葡萄中分离出的主要活性成分。已证明DHM具有多种药理活性，包括抗炎作用。然而，DHM在哮喘治疗中的潜在作用仍有待阐明。本研究旨在探讨其在过敏性哮喘小鼠中的抗炎特性。用卵清蛋白（OVA）对C57BL/6小鼠进行致敏和激发以诱导哮喘。在OVA激发前1小时给予DHM或磷酸盐缓冲盐水处理。通过酶联免疫吸附测定（ELISA）测量支气管肺泡灌洗（BAL）液中白细胞介素（IL）-4、IL-5和IL-13的水平，并且也通过ELISA测定OVA特异性血清IgE和IgG1水平。进行组织病理学染色以评估炎性细胞向BAL液、肺组织的浸润以及杯状细胞增生情况。DHM处理显著减少了BAL液中炎性细胞的总数，包括嗜酸性粒细胞、中性粒细胞、淋巴细胞和巨噬细胞。DHM还降低了BAL液中IL-4、IL-5和IL-13的水平，并减少了血清中OVA特异性IgE和IgG1的分泌。组织学染色表明，DHM处理有效地抑制了OVA诱导的肺组织炎性细胞以及气道中杯状细胞过度分泌的黏液中的炎性细胞。这些结果表明，DHM在OVA诱导的哮喘小鼠模型中具有强大的抗炎作用，具有作为治疗哮喘的抗炎剂的潜力。
