Atasilp Chalirmporn, Chansriwong Pichai, Sirachainan Ekapob, Reungwetwattana Thanyanan, Puangpetch Apichaya, Prommas Santirhat, Sirilerttrakul Suwannee, Rerkarmnuaychoke Budsaba, Wongwaisayawan Sansanee, Sukasem Chonlaphat
Division of Pharmacogenomics and Personalized Medicine, Department of Pathology, Faculty of Medicine Ramathibodi Hospital, Mahidol University, Bangkok, Thailand.
Laboratory for Pharmacogenomics, Clinical Pathology, Somdetch Phra Debharatana Medical Centre, Ramathibodi Hospital, Bangkok, Thailand.
J Clin Lab Anal. 2018 Jan;32(1). doi: 10.1002/jcla.22217. Epub 2017 Apr 10.
Irinotecan (CPT-11) is chemotherapy used mainly in the metastatic colorectal cancer. The purpose of this study was to develop and validate the LC-MS/MS for the simultaneous determination of CPT-11, SN-38, and SN-38G.
A 100 μL of plasma was prepared after protein precipitation and analyzed on a C18 column using 0.1% acetic acid in water and 0.1% acetic acid in acetonitrile as mobile phases. The mass spectrometer worked with multiple reaction monitoring (MRM) in positive scan mode. The standard curves were linear on a concentration range of 5-10 000 ng/mL for CPT-11, 5-1000 ng/mL for SN-38, and 8-1000 ng/mL for SN-38G.
In this assay, the intra and interday precision consisted of ≤9.11% and ≤11.29% for CPT-11, ≤8.70% and 8.31% for SN-38, and ≤9.90 and 9.64% for SN-38G.
This method was successfully used to quantify CPT-11, SN-38, and SN-38G and applied to a pharmacokinetic study.
伊立替康（CPT-11）是主要用于转移性结直肠癌的化疗药物。本研究的目的是开发并验证用于同时测定CPT-11、SN-38和SN-38G的液相色谱-串联质谱法。
经蛋白沉淀后制备100μL血浆，并在C18柱上进行分析，流动相为0.1%乙酸水溶液和0.1%乙酸乙腈溶液。质谱仪在正离子扫描模式下采用多反应监测（MRM）。CPT-11在5-10000 ng/mL浓度范围内、SN-38在5-1000 ng/mL浓度范围内、SN-38G在8-1000 ng/mL浓度范围内的标准曲线呈线性。
在本测定中，CPT-11的日内和日间精密度分别≤9.11%和≤11.29%，SN-38的日内和日间精密度分别≤8.70%和8.31%，SN-38G的日内和日间精密度分别≤9.90%和9.64%。
该方法成功用于定量CPT-11、SN-38和SN-38G，并应用于一项药代动力学研究。
