嗜热脂肪芽孢杆菌Y406中限制性内切酶BstYI的分离与鉴定
Isolation and characterization of restriction endonuclease BstYI from Bacillus stearothermophilus Y406.
作者信息
Chen Z F, Kong H M
机构信息
Institute of Genetics, Fudan University, Shanghai, P.R. China.
出版信息
FEBS Lett. 1988 Jul 4;234(1):169-71. doi: 10.1016/0014-5793(88)81326-7.
PMID:2839359
Abstract
BstYI, an isoschizomer of XhoII and MflI, has been purified from Bacillus stearothermophilus Y406. This enzyme recognized 5'...Pu/GATCPy...3' in DNA and cleaved between Pu and G in this sequence. BstYI can be easily isolated and purified by heparin-agarose column chromatography in a high yield (8000 units BstYI can be obtained per g wet wt of cells).
摘要
BstYI是XhoII和MflI的同裂酶，已从嗜热脂肪芽孢杆菌Y406中纯化得到。该酶识别DNA中的5'...Pu/GATCPy...3'，并在此序列中的Pu和G之间切割。通过肝素-琼脂糖柱层析可以轻松地以高产率分离和纯化BstYI（每克湿重细胞可获得8000单位BstYI）。
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