[嗜热脂肪芽孢杆菌4.4中位点特异性内切酶Bst 4.4I对识别位点的一种新型切割方式]
[A new type of cleavage of the recognition site by the site-specific endonuclease Bst 4.4I from Bacillus stearothermophilus 4.4].
作者信息
Kramarov V M, Fomenkov A I, Matvienko N I
出版信息
Bioorg Khim. 1988 Jul;14(7):916-20.
PMID:2847759
Abstract
A site-specific endonuclease Bst 4.4I was isolated from the cell extract of Bacillus stearothermophilus 4.4 and partially purified by chromatography on Ultragel AcA-44 and heparin-Sepharose. It was shown that the endonuclease cleaves lambda and M13 DNA yielding distinct fragments just as endonucleases of II and III types but, in contrast to them can produce two two-strand cuts separated with 30 to 32 nucleotides in the region of the recognition site.
摘要
一种位点特异性内切酶Bst 4.4I是从嗜热脂肪芽孢杆菌4.4的细胞提取物中分离出来的，并通过在Ultragel AcA - 44和肝素 - 琼脂糖上进行层析进行了部分纯化。结果表明，该内切酶切割λ和M13 DNA产生独特的片段，就像II型和III型内切酶一样，但与它们不同的是，它可以在识别位点区域产生两个相隔30至32个核苷酸的双链切口。
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