Prozzi D, Crichton R R, Davison J
Unit of Biochemistry, Université Catholique de Louvain, Louvain-la-Neuve, Belgium.
FEBS Lett. 1988 Jul 4;234(1):61-4. doi: 10.1016/0014-5793(88)81303-6.
The natural human H-chain ferritin was expressed in E. coli using a multi-copy expression vector containing the lambda pL promoter. A variant H-ferritin, having an altered N-terminus, was also produced. These proteins are overproduced (greater than 30% of the soluble protein), correctly assembled into its 24-subunit shell, and able to bind iron. The identity of the products was confirmed using an antibody specific for H-ferritin.
使用含有λ pL启动子的多拷贝表达载体在大肠杆菌中表达天然人H链铁蛋白。还产生了一种N端改变的变体H铁蛋白。这些蛋白质过量表达（超过可溶性蛋白的30%），正确组装成其24亚基外壳，并能够结合铁。使用针对H铁蛋白的特异性抗体确认了产物的身份。
