Marguet D, Fantino E, Lauquin G J
Laboratoire de Physiologie Cellulaire, Faculté des Sciences de Marseille-Luminy, Université d'Aix-Marseille, France.
FEBS Lett. 1988 Jul 18;234(2):421-5. doi: 10.1016/0014-5793(88)80129-7.
We have isolated and purified chromosome XV DNA molecules from the yeast S. cerevisiae using contour-clamped homogeneous electric field (CHEF) gel electrophoresis. A chromosome-specific mini-library was constructed and the element of the SRP1 (serine-rich protein) related sequence family located within chromosome XV was isolated by in situ colony hybridization with an SRP1 probe. Results indicated that (i) a single-copy sequence homologous to SRP1 is present within chromosome XV; (ii) this sequence lies within the 2.3 kb HindIII fragment of the plasmid pXVAc6; (iii) the lack of a specific transcript from this SRP1-related element suggests that it could be considered as a pseudogene.
