酿酒酵母中的PHO80/TUP7基因座位于第十五号染色体的左臂：通过染色体工程进行定位。
The PHO80/TUP7 locus in Saccharomyces cerevisiae is on the left arm of chromosome XV: mapping by chromosome engineering.
作者信息
Kawasaki H, Matsuzaki H, Nakajima R, Oshima Y
机构信息
Department of Biotechnology, Faculty of Engineering, Osaka University, Japan.
出版信息
Yeast. 1991 Nov;7(8):859-65. doi: 10.1002/yea.320070812.
PMID:1789008
Abstract
The PHO80/TUP7 locus in Saccharomyces cerevisiae is reported to be located on the right arm of chromosome XV close to its centromere. In the present study, the locus has been reassigned to the left arm of the same chromosome by reciprocal recombination between chromosomes V and XV at URA3 (on chromosome V) and PHO80/TUP7 loci by using the site-specific recombination system of the yeast plasmid pSR1.
摘要
据报道，酿酒酵母中的PHO80/TUP7基因座位于第十五号染色体右臂靠近着丝粒的位置。在本研究中，利用酵母质粒pSR1的位点特异性重组系统，通过五号染色体和十五号染色体在URA3（位于五号染色体）和PHO80/TUP7基因座处的相互重组，该基因座已被重新定位到同一染色体的左臂。
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