Karpel R, Olami Y, Taglicht D, Schuldiner S, Padan E
Department of Molecular and Microbial Ecology, Hadassah Medical School, Hebrew University, Jerusalem, Israel.
J Biol Chem. 1988 Jul 25;263(21):10408-14.
By introduction of deletions and/or insertions, we have defined a small DNA fragment (1.58 kilobase pairs) which contains the ant gene. Thus, transformants of all plasmid constructs containing this segment exhibit Antup phenotype, i.e. growth is Li+-resistant and Na+/H+ antiporter activity is increased in isolated everted membrane vesicles. Utilizing the T7 promoter expression system, we also found that this fragment encodes for a single protein of 35 kDa. The DNA fragment has been sequenced and found to contain an open reading frame of 1085 base pairs. Analysis of the sequence of the predicted protein suggests the presence of 10 putative transmembrane segments in the protein.
通过引入缺失和/或插入，我们确定了一个包含ant基因的小DNA片段（1.58千碱基对）。因此，所有含有该片段的质粒构建体的转化体均表现出Antup表型，即生长对Li + 具有抗性，并且在分离的外翻膜囊泡中Na + /H + 反向转运蛋白活性增加。利用T7启动子表达系统，我们还发现该片段编码一种35 kDa的单一蛋白质。该DNA片段已被测序，发现含有一个1085个碱基对的开放阅读框。对预测蛋白质序列的分析表明该蛋白质中存在10个推定的跨膜区段。
