大肠杆菌钠氢反向转运蛋白基因的特性分析
Characterization of a Na+/H+ antiporter gene of Escherichia coli.
作者信息
Goldberg E B, Arbel T, Chen J, Karpel R, Mackie G A, Schuldiner S, Padan E
出版信息
Proc Natl Acad Sci U S A. 1987 May;84(9):2615-9. doi: 10.1073/pnas.84.9.2615.
Abstract
A mutant of Escherichia coli with increased Na+/H+ antiport activity was isolated and found by other workers to harbor two mutations [Niiya, S., Yamasaki, K., Wilson, T.H. & Tsuchiya, T. (1982) J. Biol. Chem. 257, 8902-8906]. The mutation that leads to increased Na+/H+ antiport (antup) has now been separated and mapped. antup maps in the vicinity of 0.5 min on the E. coli map, and the presence of this mutation alone in an isogenic pair raises antiport activity (Vmax) by approximately 4-fold. We also characterized cells hearing plasmids containing fragments of a 15-kilobase-pair segment of DNA between carA and dnaJ. A wild-type gene located within 2 kilobase pairs counterclockwise of rpsT increases the Na+/H+ antiport activity when present in multiple copies.
摘要
分离出一株具有增强的Na⁺/H⁺反向转运活性的大肠杆菌突变体，其他研究人员发现该突变体存在两个突变[Niiya, S., Yamasaki, K., Wilson, T.H. & Tsuchiya, T. (1982) J. Biol. Chem. 257, 8902 - 8906]。现在已经分离并定位了导致Na⁺/H⁺反向转运增加（antup）的突变。antup位于大肠杆菌基因图谱上约0.5分钟处，在同基因对中单独存在该突变会使反向转运活性（Vmax）提高约4倍。我们还对携带含有carA和dnaJ之间15千碱基对DNA片段的质粒的细胞进行了表征。位于rpsT逆时针方向2千碱基对范围内的一个野生型基因，当以多拷贝形式存在时会增加Na⁺/H⁺反向转运活性。
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