源自人心脏外膜细胞的两条诱导多能干细胞系的产生与鉴定
Generation and characterization of two iPSC lines from human epicardium-derived cells.
作者信息
Paulitschek Christina, Schulze-Matz Peggy, Hesse Julia, Schmidt Timo, Wruck Wasco, Adjaye James, Schrader Jürgen
机构信息
Department of Molecular Cardiology, Medical Faculty, Heinrich Heine University, 40225 Duesseldorf, Germany.
Institute for Stem Cell Research and Regenerative Medicine, Medical Faculty, Heinrich Heine University, 40225 Duesseldorf, Germany.
出版信息
Stem Cell Res. 2017 Apr;20:50-53. doi: 10.1016/j.scr.2017.02.007. Epub 2017 Feb 24.
Human epicardium-derived cells (EPDC) were reprogrammed to generate two iPSC lines, MCDU1i-EPDC and MCDU2i-EPDC, by nucleofection of episomal-based plasmids expressing the reprogramming factors OCT4, SOX2, KLF4, c-MYC, NANOG and LIN28. Pluripotency was confirmed in vitro by immunofluorescence analysis and embryoid body formation. The iPSC lines and the human embryonic stem cell line H1 show a Pearson correlation co-efficient of 0.951 (MCDU1i-EPDC) and 0.937 (MCDU2i-EPDC) as assessed by comparative transcriptome profiling.
通过电穿孔法将表达重编程因子OCT4、SOX2、KLF4、c-MYC、NANOG和LIN28的游离型质粒导入人心脏外膜来源细胞（EPDC），从而将其重编程，生成了两个诱导多能干细胞系，即MCDU1i-EPDC和MCDU2i-EPDC。通过免疫荧光分析和胚状体形成在体外证实了多能性。通过比较转录组分析评估，诱导多能干细胞系与人类胚胎干细胞系H1的皮尔逊相关系数分别为0.951（MCDU1i-EPDC）和0.937（MCDU2i-EPDC）。
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