Kawala Marie-Ann, Bohndorf Martina, Graffmann Nina, Wruck Wasco, Zatloukal Kurt, Adjaye James
Institute for Stem Cell Research and Regenerative Medicine, Medical Faculty, Heinrich Heine University, 40225 Düsseldorf, Germany.
Institute of Pathology, Medical University of Graz, 8036 Graz, Austria.
Stem Cell Res. 2016 Nov;17(3):597-599. doi: 10.1016/j.scr.2016.10.002. Epub 2016 Oct 19.
Primary fibroblasts from a healthy 19years old female were reprogrammed by transduction of retroviruses OCT4, SOX2, c-MYC and KLF4. iPSCs were characterized by immunocytochemistry, embryonic body-formation, DNA-fingerprint and karyotype analysis and comparative transcriptome analyses with the human embryonic stem cell line H1 revealed a Pearsons correlation coefficient of 0.8952.
通过逆转录病毒OCT4、SOX2、c-MYC和KLF4转导，对一名19岁健康女性的原代成纤维细胞进行重编程。通过免疫细胞化学、胚体形成、DNA指纹图谱和核型分析对诱导多能干细胞进行表征，并且与人类胚胎干细胞系H1进行比较转录组分析，结果显示皮尔逊相关系数为0.8952。
