Deng Yiqin, Ding Xiongqi, Huang Xiaochun, Yang Yiying, Chen Chang
Key Laboratory of Tropical Marine Bio-resources and Ecology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou, China; Guangdong Provincial Key Laboratory of Applied Marine Biology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou, China; University of Chinese Academy of Sciences, Beijing, China.
Key Laboratory of Tropical Marine Bio-resources and Ecology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou, China; Guangdong Provincial Key Laboratory of Applied Marine Biology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou, China; Xisha/Nansha Ocean Observation and Research Station, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou, China.
Mar Genomics. 2017 Oct;35:23-26. doi: 10.1016/j.margen.2017.03.006. Epub 2017 Apr 5.
Branched-chain amino acids (BCAAs) play important roles in nitrogen metabolism. However, little is known about the metabolism of BCAAs in the fish pathogen Vibrio alginolyticus. In this study, the global gene expression patterns of V. alginolyticus ZJ-T cultured in M63 minimal medium supplemented with ammonium sulfate or with three BCAAs (isoleucine, leucine and valine) as nitrogen source were evaluated by transcriptome analysis. The results revealed that 311 genes are up-regulated (|log(Fold Change)|>1), which are involved in the pathways of flagellar assembly, bacterial chemotaxis and oxidative phosphorylation etc, and meanwhile 251 genes are down-regulated, which are involved in the pathways of BCAAs biosynthesis, selenocompound metabolism and C5-branced dibasic acid metabolism etc. This study contributes to the understanding of the BCAAs metabolism in the Vibrios.
支链氨基酸（BCAAs）在氮代谢中发挥着重要作用。然而，关于鱼类病原体溶藻弧菌中BCAAs的代谢情况却知之甚少。在本研究中，通过转录组分析评估了在添加硫酸铵或三种BCAAs（异亮氨酸、亮氨酸和缬氨酸）作为氮源的M63基本培养基中培养的溶藻弧菌ZJ-T的全局基因表达模式。结果显示，有311个基因上调（|log(倍数变化)|>1），这些基因参与鞭毛组装、细菌趋化性和氧化磷酸化等途径，同时有251个基因下调，这些基因参与BCAAs生物合成、硒化合物代谢和C5-支链二元酸代谢等途径。本研究有助于了解弧菌中BCAAs的代谢情况。
