Aayadi Hoda, Mittal Smriti P K, Deshpande Anjali, Gore Makarand, Ghaskadbi Saroj S
Department of Zoology, Savitribai Phule Pune University, Pune 411007, India.
AJ Organica Pvt. Ltd., Pune 411057, India.
Biochem Biophys Res Commun. 2017 May 20;487(1):62-67. doi: 10.1016/j.bbrc.2017.04.013. Epub 2017 Apr 7.
Geraniin is a hydrolysable tannin, widely present in many plant species, specifically used in traditional medicines. It has been shown to exhibit strong antioxidant activity in vitro. This study was performed to investigate hepatoprotective activity of geraniin against carbon tetrachloride (CCl) induced damage in Swiss albino mice. Mice were treated with 30 and 60 mg/kg geraniin for 10 days followed by CCl administration for 24 h. Increase in Serum biochemical marker enzymes and histological deteriorative changes of liver tissue after CCl administration were attenuated by geraniin. Geraniin significantly reduced CCl induced lipid peroxidation, increase in amount of glutathione, glutathione reductase and Heme oxygenase-1 (HO-1). On the other hand it inhibited significant reduction in catalase activity and expression caused by CCl administration. Pre-treatment with geraniin reduced phosphorylation of translation initiation factor eIF2α, at serine 51, caused by CCl exposure and reduced elevated expression of its upstream kinase, Heme-regulated Inhibitor (HRI). These results clearly demonstrate hepatoprotective activity of geraniin against CCl-induced acute hepatotoxicity via its free radical scavenging and antioxidant activities.
老鹳草素是一种可水解单宁，广泛存在于许多植物物种中，特别用于传统医学。体外实验表明它具有很强的抗氧化活性。本研究旨在探讨老鹳草素对四氯化碳（CCl）诱导的瑞士白化小鼠肝损伤的保肝活性。小鼠分别用30和60mg/kg老鹳草素处理10天，随后给予CCl 24小时。老鹳草素减轻了CCl给药后血清生化标志物酶的升高以及肝组织的组织学恶化变化。老鹳草素显著降低了CCl诱导的脂质过氧化，增加了谷胱甘肽、谷胱甘肽还原酶和血红素加氧酶-1（HO-1）的含量。另一方面，它抑制了CCl给药引起的过氧化氢酶活性和表达的显著降低。老鹳草素预处理降低了CCl暴露引起的翻译起始因子eIF2α在丝氨酸51处的磷酸化，并降低了其上游激酶血红素调节抑制剂（HRI）的表达升高。这些结果清楚地证明了老鹳草素通过其自由基清除和抗氧化活性对CCl诱导的急性肝毒性具有保肝活性。
