Furkan Mohammad, Rizvi Asim, Alam Md Tauqir, Zaman Masihuz, Khan Rizwan Hasan, Naeem Aabgeena
Department of Biochemistry, Faculty of Life Sciences, Aligarh Muslim University, Aligarh 202002, India.
Interdisciplinary Biotechnology Unit, Aligarh Muslim University, Aligarh 202002, India.
Int J Biol Macromol. 2017 Sep;102:893-900. doi: 10.1016/j.ijbiomac.2017.04.026. Epub 2017 Apr 7.
Importance of cytochrome c arises from its involvement in apoptosis, sequence homology and conserveness during molecular evolution. Dopamine at 25μM concentration for 30h resulted in cytochrome c aggregation as evident by increase in ANS and ThT binding, structural transition from α helix to β sheet and shift in congo red assay. Interestingly, serotonin at 25μM concentration incubated for 60h abrogates the aggregatory effect on cytochrome c. Fibrillation of human cytochrome c in the presence of dopamine gives the clue about conformational changes taking place in protein and example of natural aggregation and inhibitory effect of serotonin gives the clue about natural defence mechanism occurring in human body.
细胞色素c的重要性源于其参与细胞凋亡、分子进化过程中的序列同源性和保守性。25μM浓度的多巴胺作用30小时导致细胞色素c聚集，这可通过ANS和ThT结合增加、从α螺旋到β折叠的结构转变以及刚果红试验中的位移得以证明。有趣的是，25μM浓度的血清素孵育60小时可消除对细胞色素c的聚集作用。多巴胺存在下人类细胞色素c的纤维化揭示了蛋白质中发生的构象变化，而血清素的自然聚集和抑制作用的例子则揭示了人体中发生的自然防御机制。
