Żywicka Bogusława, Szymonowicz Maria, Bryła Danuta, Rybak Zbigniew
Department of Experimental Surgery and Biomaterials Research, Wroclaw Medical University, Wroclaw, Poland.
Polim Med. 2016 Jul-Dec;46(2):135-143. doi: 10.17219/pim/68618.
The development of technologies and scientific disciplines connected with medical implantation devices is dynamically affecting modern treatments by contemporary medicine and veterinary medicine; it also entails a need to monitor their impact on living organisms.
The aim of the study was to conduct a comparative histological evaluation of the response of soft tissues after implanting monofilament fibers from resorbable glyconate and from non-resorbable polypropylene (PP) and polyamide (PA) in rats.
Non-resorbable polyamide-based fibers were applied to skin anastomoses in rats. Macroscopic and histological evaluations were performed on the 7th, 14th and 30th days. Non-resorbable polypropylene fibers and resorbable glyconate fibers (composed of 72% glycolide, 14% trimethylene carbonate and 4% caprolactone) were implanted in muscle tissue for periods of 7, 14, 30 and 90 days.
A semi-quantitative and qualitative histological evaluation found different dynamics and degrees of intensification of cell and tissue response around the resorbable and non-resorbable fibers being tested. The resorption process of the glyconate threads caused a prolonged inflammatory cellular response compared to the non-resorbable threads; it passed, however, without the participation of giant cells. Around the non-resorbable threads the observed cellular response was less intensified, with the formation of single polymorphonuclear macrophages around the PP threads, along with a stronger degree of fibrosis and the presence of fatty infiltrate.
During the early period, moderately intensified inflammatory cell response with the presence of single giant cells was observed around the non-degradable PA and PP fibers. In the late period, a band of fibrous connective tissue was present around the PP threads. Glyconate fibers underwent fragmentation and the process of resorption, which was associated with a weakly intensified inflammatory process lasting up to 90 days after implantation.
与医用植入装置相关的技术和科学学科的发展正在动态影响当代医学和兽医学的现代治疗方法；这也需要监测它们对生物体的影响。
本研究的目的是对大鼠植入可吸收糖酸盐和不可吸收聚丙烯（PP）及聚酰胺（PA）单丝纤维后软组织的反应进行比较组织学评估。
将不可吸收的聚酰胺基纤维应用于大鼠的皮肤吻合处。在第7天、第14天和第30天进行宏观和组织学评估。将不可吸收的聚丙烯纤维和可吸收的糖酸盐纤维（由72%乙交酯、14%碳酸三亚甲酯和4%己内酯组成）植入肌肉组织7天、14天、30天和90天。
半定量和定性组织学评估发现，在测试的可吸收和不可吸收纤维周围，细胞和组织反应的动态变化和强化程度不同。与不可吸收的线相比，糖酸盐线的吸收过程导致炎症细胞反应延长；然而，该过程在没有巨细胞参与的情况下进行。在不可吸收的线周围，观察到的细胞反应强化程度较低，在PP线周围形成单个多形核巨噬细胞，同时纤维化程度更强且存在脂肪浸润。
在早期，在不可降解的PA和PP纤维周围观察到炎症细胞反应适度强化且存在单个巨细胞。在后期，PP线周围出现纤维结缔组织带。糖酸盐纤维发生碎裂和吸收过程，这与植入后长达90天的炎症过程轻微强化有关。
