Liu Ding-Hua, Yao Yun-Tai, Li Li-Huan, Huang Chun-Mei
Clin Lab. 2017 Apr 1;63(4):833-838. doi: 10.7754/Clin.Lab.2016.160908.
The study aimed to investigate the influence of in vitro storage on erythrocyte complement receptor one (E-CR1), cell shrinkage and eryptosis of human red blood cells (RBCs), and to assess the possible effects of ulinastatin (UTI) on them.
After collection, RBCs were treated with saline (control group) and different concentrations of UTI (5,000 U/mL, 10,000 U/mL, and 50,000 U/mL in Group C1, Group C2, and Group C3, respectively). E-CR1, cell size, and phosphatidylserine (PS) exposure and intracellular Ca2+ concentration were analyzed by flow cytometer every 7 days up to Day 35.
E-CR1 level and cell size of all groups decreased during storage. In the control group, E-CR1 began to decrease on Day 28 and cells shrank on Day 21. The E-CR1 level of Group C2 was significantly higher than that of the control group beginning on Day 21. The cells of Group C1 and Group C2 began to shrink remarkably on Day 21, and those of Group C3 on Day 35. PS-exposure levels of 4 groups started to increase on Day 7 (p < 0.05), while from Day 14 to 35 those of Group C3 were significantly lower than the control group (p < 0.05). The intracellular Ca2+ levels of the control group started to increase significantly on Day 7, one week earlier than the experimental groups. From Day 21 to 35, the intracellular Ca2+ levels of Group C2 and C3 were significantly lower than those of the control group (p < 0.05).
RBCs underwent E-CR1 loss, cell shrinkage, and eryptosis during in vitro storage, which could be attenuated by UTI.
本研究旨在探讨体外储存对人红细胞（RBC）的红细胞补体受体1（E-CR1）、细胞皱缩和红细胞凋亡的影响，并评估乌司他丁（UTI）对其可能的作用。
采集后，RBC分别用生理盐水（对照组）和不同浓度的UTI（C1组、C2组和C3组分别为5000 U/mL、10000 U/mL和50000 U/mL）处理。直至第35天，每7天用流式细胞仪分析E-CR1、细胞大小、磷脂酰丝氨酸（PS）暴露和细胞内Ca2+浓度。
储存期间所有组的E-CR1水平和细胞大小均降低。对照组中，E-CR1在第28天开始下降，细胞在第21天开始皱缩。从第21天开始，C2组的E-CR1水平显著高于对照组。C1组和C2组的细胞在第21天开始明显皱缩，C3组的细胞在第35天开始皱缩。4组的PS暴露水平在第7天开始升高（p<0.05），而从第14天到35天，C3组的PS暴露水平显著低于对照组（p<0.05）。对照组的细胞内Ca2+水平在第7天开始显著升高，比实验组早一周。从第21天到35天，C2组和C3组的细胞内Ca2+水平显著低于对照组（p<0.05）。
RBC在体外储存期间经历了E-CR1丢失、细胞皱缩和红细胞凋亡，UTI可减轻这些变化。
