Palacios J M, Pazos A, Dietl M M, Schlumpf M, Lichtensteiger W
Preclinical Research, SANDOZ Ltd., Basle, Switzerland.
Neuroscience. 1988 Apr;25(1):307-17. doi: 10.1016/0306-4522(88)90028-0.
Using in vitro receptor autoradiographic techniques we have analysed the pre- and postnatal development of neurotensin receptors in the rat brain. Receptors were labeled with [3H] or [125I]neurotensin in mounted tissue sections from animals of ages gestational day 14 until the postnatal day 21 as well as young adult animals. Very low densities of neurotensin receptors were visualized on gestational days 14 and 15. Between gestational days 16 and 18 a marked increase in the density of neurotensin receptors was seen in the developing neocortex. Densities in other brain areas, particularly the midbrain and brainstem were much lower than cortical densities. The density of neurotensin receptors in the cortex increased through the last part of the gestation and early postnatal life until it peaked at the end of the first postnatal week. After that, neurotensin receptor binding decreased dramatically reaching lower densities seen in the adult animal at the end of the third postnatal week. Development of neurotensin receptors in other brain areas followed very different time patterns. Neurotensin receptors in the midbrain were seen first at gestational day 18 and increased slowly with development to reach adult levels at about the second week of postnatal life. Neurotensin receptors in the hippocampal formation demonstrated postnatal development; they were detected at postnatal day 5 and showed a developmental peak around the second week. These patterns were seen with both 3H- and 125I-labeled neurotensin, thus excluding possible differential quenching artifacts. These clear differential regional ontogenetic patterns for neurotensin receptors are the main findings of these experiments. The very high densities present in the cortex even in fetal stages suggest that neurotensin could play a role in the development of the brain.
我们运用体外受体放射自显影技术，分析了大鼠脑中神经降压素受体在产前和产后的发育情况。使用[3H]或[125I]神经降压素标记来自妊娠第14天直至出生后第21天的动物以及成年动物的固定组织切片中的受体。在妊娠第14天和15天，可见神经降压素受体的密度非常低。在妊娠第16天至18天之间，发育中的新皮质中神经降压素受体的密度显著增加。其他脑区，特别是中脑和脑干的密度远低于皮质密度。皮质中神经降压素受体的密度在妊娠后期和出生后早期持续增加，直至在出生后第一周结束时达到峰值。此后，神经降压素受体结合显著下降，在出生后第三周结束时降至成年动物所见的较低密度。其他脑区神经降压素受体的发育遵循非常不同的时间模式。中脑中的神经降压素受体在妊娠第18天首次出现，并随着发育缓慢增加，在出生后约第二周达到成年水平。海马结构中的神经降压素受体表现出产后发育；它们在出生后第5天被检测到，并在第二周左右出现发育高峰。使用3H和125I标记的神经降压素均观察到这些模式，从而排除了可能的差异淬灭假象。这些神经降压素受体明显的区域发育差异模式是这些实验的主要发现。即使在胎儿阶段，皮质中存在的非常高的密度表明神经降压素可能在脑发育中起作用。
