Beier Sebastian, Thiel Thomas, Münch Thomas, Scholz Uwe, Mascher Martin
Leibniz Institute of Plant Genetics and Crop Plant Research (IPK) Gatersleben, Corrensstr. 3, 06466 Seeland, Germany.
KWS Saat SE, Grimsehlstr. 31, 37555 Einbeck, Germany.
Bioinformatics. 2017 Aug 15;33(16):2583-2585. doi: 10.1093/bioinformatics/btx198.
Microsatellites are a widely-used marker system in plant genetics and forensics. The development of reliable microsatellite markers from resequencing data is challenging.
We extended MISA, a computational tool assisting the development of microsatellite markers, and reimplemented it as a web-based application. We improved compound microsatellite detection and added the possibility to display and export MISA results in GFF3 format for downstream analysis.
MISA-web can be accessed under http://misaweb.ipk-gatersleben.de/. The website provides tutorials, usage note as well as download links to the source code.
微卫星是植物遗传学和法医学中广泛使用的标记系统。从重测序数据中开发可靠的微卫星标记具有挑战性。
我们扩展了MISA（一种辅助微卫星标记开发的计算工具），并将其重新实现为基于网络的应用程序。我们改进了复合微卫星检测，并增加了以GFF3格式显示和导出MISA结果以便进行下游分析的功能。
可通过http://misaweb.ipk-gatersleben.de/访问MISA-web。该网站提供教程、使用说明以及源代码的下载链接。
