Mahmoodi Khalil, Kamali Koorosh, Karami Elham, Soltanpour Mohammad Soleiman
Department of Cardiology, School of Medicine, Zanjan University of Medical Sciences, Zanjan, Iran.
Department of Public Health, School of Public Health, Zanjan University of Medical Sciences, Zanjan, Iran.
J Res Med Sci. 2017 Jan 27;22:8. doi: 10.4103/1735-1995.199088. eCollection 2017.
Matrix metalloproteinase 9 (MMP9) -1562C>T (rs3918242) polymorphism has been proposed as a risk factor for coronary artery disease (CAD) with conflicting results. The aim of the present study was to investigate the association of -1562C>T genetic polymorphism, gene expression and circulating levels of MMP9 with CAD risk in an Iranian subpopulation in in Zanjan City.
This was a retrospective case-control study we investigated retrospectively 100 patients with angiographically verified CAD and 100 matched controls. Genotyping of -1562C>T polymorphism was done by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). Gene expression levels and circulating levels of MMP9 was determined by real-time reverse transcription-PCR and enzyme immunoassay method, respectively. Statistical analysis was done using Student's -test or Chi-square test by SPSS 16 software.
The mean circulating levels of MMP9 were significantly higher in CAD Group than control group ( = 0.002). Mean plasma levels of MMP9 were also significantly higher in triple vessel stenosis patients than double vessel or single vessel stenosis patients ( < 0.001). Moreover, mean plasma levels and gene expression levels of MMP9 were significantly higher in T allele carrier than C allele carrier of MMP9 -1562C>T polymorphism ( = 0.002, = 0.01, respectively). However, genotype and allele frequencies of MMP9 -1562C>T polymorphism were similar between CAD patients and controls ( > 0.05). Additionally, the -1562C>T polymorphism of MMP9 gene didn't increase the risk of CAD in dominant ( = 0.537) or recessive ( = 0.249) genetic models.
Our study demonstrated that circulating levels of MMP9 but not -1562C>T polymorphism of MMP9 gene may be a risk factor for development and severity of CAD in an Iranian subpopulation in Zanjan.
基质金属蛋白酶9（MMP9）-1562C>T（rs3918242）多态性被认为是冠状动脉疾病（CAD）的一个危险因素，但其结果存在争议。本研究的目的是调查赞詹市伊朗亚人群中MMP9 -1562C>T基因多态性、基因表达及循环水平与CAD风险的相关性。
这是一项回顾性病例对照研究，我们回顾性调查了100例经血管造影证实的CAD患者和100例匹配的对照。采用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）方法对-1562C>T多态性进行基因分型。分别采用实时逆转录-PCR和酶免疫分析法测定MMP9的基因表达水平和循环水平。使用SPSS 16软件进行统计学分析，采用Student's t检验或卡方检验。
CAD组MMP9的平均循环水平显著高于对照组（P = 0.002）。三支血管狭窄患者的MMP9平均血浆水平也显著高于双支血管或单支血管狭窄患者（P < 0.001）。此外，MMP9 -1562C>T多态性的T等位基因携带者的MMP9平均血浆水平和基因表达水平显著高于C等位基因携带者（分别为P = 0.002，P = 0.01）。然而，CAD患者和对照组之间MMP9 -1562C>T多态性的基因型和等位基因频率相似（P > 0.05）。此外，MMP9基因的-1562C>T多态性在显性（P = 0.537）或隐性（P =
