Rajput Y S, Gupta M N
Chemistry Department, Indian Institute of Technology, Hauz Khas, New Delhi.
Biotechnol Appl Biochem. 1988 Jun;10(3):242-50.
Hybrid enzymes which have two different enzyme activities linked together covalently may be useful reagents for various applications, such as the determination of complex biological structures. The present paper describes the preparation and purification of two such enzyme-enzyme conjugates, namely, trypsin-chymotrypsin and trypsin-alkaline phosphatase. Whereas the former has been prepared by using the well-known bifunctional reagent glutaraldehyde, the latter exploited the Schiff base formation between the oxidized carbohydrate moiety of alkaline phosphatase and the free amino groups of trypsin.
具有两种通过共价键连接在一起的不同酶活性的杂合酶可能是用于各种应用的有用试剂，例如用于测定复杂的生物结构。本文描述了两种这样的酶 - 酶缀合物的制备和纯化，即胰蛋白酶 - 胰凝乳蛋白酶和胰蛋白酶 - 碱性磷酸酶。前者是通过使用著名的双功能试剂戊二醛制备的，而后者则利用了碱性磷酸酶氧化的碳水化合物部分与胰蛋白酶的游离氨基之间形成席夫碱的反应。
