Jeanson A, Cloes J M, Bouchet M, Rentier B
General Biology Department, University of Liège, Belgium.
Anal Biochem. 1988 Aug 1;172(2):392-6. doi: 10.1016/0003-2697(88)90460-5.
Conjugates of alkaline phosphatase (AP) and mouse monoclonal immunoglobulins G (IgG) were prepared by means of the heterobifunctional linker, N-succinimidyl 3-(2-pyridyldithio)-propionate. The efficiency of such conjugates can be improved by optimizing the degree of substitution of IgG and AP. We have determined conditions yielding better performing conjugates than those synthesized by methods described previously. Moreover, the results obtained with the technique presented here are quite reproducible with all four monoclonal antibodies tested.
通过异双功能连接剂N-琥珀酰亚胺基-3-(2-吡啶二硫基)丙酸酯制备了碱性磷酸酶（AP）与小鼠单克隆免疫球蛋白G（IgG）的缀合物。通过优化IgG和AP的取代度，可以提高此类缀合物的效率。我们已经确定了一些条件，这些条件所产生的缀合物比以前描述的方法合成的缀合物性能更好。此外，用这里介绍的技术获得的结果在所有四种测试的单克隆抗体中都具有相当高的可重复性。
