Fuchs B B, Avrova N F, Starosvetskaya N A, Boltovskaya M N, Zaraisky E I, Karpova O B, Reznikova E V, Soldatova L N, Stepanov A A
Institute of Human Morphology, USSR Academy of Medical Sciences, Moscow.
Biotechnol Appl Biochem. 1988 Jun;10(3):273-86.
Immunization of mice with a pure preparation of the ganglioside adsorbed on Salmonella typhimurium and hybridization of splenocytes with myeloma P3-X63-Ag 8.653 have resulted in hybridomas producing monoclonal antibodies against ganglioside Fuc GM1, a marker of human small cell lung carcinoma. Characterization of four hybridomal clones and data on the antigenic specificity of the monoclonal antibodies are given. All four monoclonal antibodies reacted only with Fuc GM1 in an enzyme-linked immunosorbent assay. In radioimmunodetection of the antigen on thin-layer plates, two of the four monoclonal antibodies gave cross-reactions with Fuc GD1b. The obtained monoclonal antibodies have revealed the presence of Fuc GM1 in all seven cases of small cell lung carcinoma we have studied and the absence of Fuc GM1 in the normal human lung tissue and in lung adenocarcinomas.
用吸附在鼠伤寒沙门氏菌上的神经节苷脂纯制剂免疫小鼠，并使脾细胞与骨髓瘤P3-X63-Ag 8.653杂交，已产生了针对神经节苷脂Fuc GM1（一种人类小细胞肺癌标志物）的单克隆抗体的杂交瘤。给出了四个杂交瘤克隆的表征以及单克隆抗体的抗原特异性数据。在酶联免疫吸附测定中，所有四种单克隆抗体仅与Fuc GM1反应。在薄层层析板上对抗原进行放射免疫检测时，四种单克隆抗体中的两种与Fuc GD1b发生交叉反应。所获得的单克隆抗体已揭示在我们研究的所有7例小细胞肺癌病例中均存在Fuc GM1，而在正常人类肺组织和肺腺癌中不存在Fuc GM1。
