Fredman P, Brezicka T, Holmgren J, Lindholm L, Nilsson O, Svennerholm L
Biochim Biophys Acta. 1986 Feb 12;875(2):316-23. doi: 10.1016/0005-2760(86)90182-7.
The binding specificity of thirteen mouse monoclonal antibodies reacting with Fuc-GM1, Fuc alpha 1-2Gal beta 1-3GalNAc beta 1-4(NeuAc alpha 2-3)-Gal beta 1-4Glc beta 1-1Cer, a ganglioside found to be associated with small cell lung carcinoma (O. Nilsson et al. (1984) Glycoconjugate J. 1, 43-49) was studied. The results are based upon radioimmunodetection of their binding to structurally related glycolipids adsorbed to microtiter plates or chromatographed on thin-layer plates. Four of thirteen antibodies reacted only with Fuc-GM1 and both the fucose and the sialic residues were necessary for binding. Optimal binding was obtained when the sialic acid was N-acetylneuraminic acid. When this sialic acid residue was substituted with N-glycoloylneuraminic acid the binding activity was reduced and up to 10-times more Fuc-GM1 was needed for detection. The ceramide composition did not influence the binding. The other nine monoclonal antibodies cross-reacted with glycolipids containing structures closely related to Fuc-GM1 and differed from the specific ones by recognizing a smaller portion of the carbohydrate moiety in Fuc-GM1. These results indicate that anticarbohydrate monoclonal antibodies, recognizing structures involving a large proportion of the sugar in the glycolipid, possess a high specificity and might be useful for detection of tumor-associated ganglioside antigen.
研究了13种与Fuc-GM1（Fucα1-2Galβ1-3GalNAcβ1-4(NeuAcα2-3)-Galβ1-4Glcβ1-1Cer，一种被发现与小细胞肺癌相关的神经节苷脂（O.尼尔森等人，(1984)《糖缀合物杂志》1, 43 - 49））反应的小鼠单克隆抗体的结合特异性。结果基于对它们与吸附在微量滴定板上或在薄层板上色谱分离的结构相关糖脂结合的放射免疫检测。13种抗体中的4种仅与Fuc-GM1反应，结合需要岩藻糖和唾液酸残基。当唾液酸为N-乙酰神经氨酸时可获得最佳结合。当该唾液酸残基被N-羟乙酰神经氨酸取代时，结合活性降低，检测所需的Fuc-GM1多达10倍。神经酰胺组成不影响结合。其他9种单克隆抗体与含有与Fuc-GM1结构密切相关的糖脂发生交叉反应，与特异性抗体的不同之处在于识别Fuc-GM1中较小部分的碳水化合物部分。这些结果表明，识别涉及神经节苷脂中大部分糖的结构的抗碳水化合物单克隆抗体具有高特异性，可能有助于检测肿瘤相关神经节苷脂抗原。
