一种基于改进的滤膜辅助样品制备的微藻蛋白质组学分析方法。

A Method for Microalgae Proteomics Analysis Based on Modified Filter-Aided Sample Preparation.

作者信息

Li Song, Cao Xupeng, Wang Yan, Zhu Zhen, Zhang Haowei, Xue Song, Tian Jing

机构信息

School of Bioengineering, Dalian Polytechnic University, Dalian, 116034, China.

Marine Bioengineering Group, Dalian Institute of Chemical Physics, CAS, Dalian, 116023, China.

出版信息

Appl Biochem Biotechnol. 2017 Nov;183(3):923-930. doi: 10.1007/s12010-017-2473-9. Epub 2017 Apr 11.

DOI:10.1007/s12010-017-2473-9

PMID:28401332

Abstract

With the fast development of microalgal biofuel researches, the proteomics studies of microalgae increased quickly. A filter-aided sample preparation (FASP) method is widely used proteomics sample preparation method since 2009. Here, a method of microalgae proteomics analysis based on modified filter-aided sample preparation (mFASP) was described to meet the characteristics of microalgae cells and eliminate the error caused by over-alkylation. Using Chlamydomonas reinhardtii as the model, the prepared sample was tested by standard LC-MS/MS and compared with the previous reports. The results showed mFASP is suitable for most of occasions of microalgae proteomics studies.

摘要

随着微藻生物燃料研究的快速发展，微藻的蛋白质组学研究迅速增加。自2009年以来，滤膜辅助样品制备（FASP）方法是一种广泛使用的蛋白质组学样品制备方法。在此，描述了一种基于改良滤膜辅助样品制备（mFASP）的微藻蛋白质组学分析方法，以适应微藻细胞的特性并消除过度烷基化导致的误差。以莱茵衣藻为模型，将制备的样品通过标准液相色谱-串联质谱（LC-MS/MS）进行检测，并与先前的报道进行比较。结果表明，mFASP适用于大多数微藻蛋白质组学研究的情况。