Cell Biology and Genetics Department, Shantou University Medical College, Shantou, Guangdong, P.R. China.
Department of Clinical Laboratory, Henan Province Hospital of Traditional Chinese Medicine, Second Affiliated Hospital of Henan University of TCM, Henan, P.R. China.
J Med Virol. 2017 Sep;89(9):1597-1605. doi: 10.1002/jmv.24829. Epub 2017 May 29.
MicroRNA (miRNA), which has been shown to correlate with liver functions, has been proposed as a new biomarker reflecting liver injury. The aim of the study was to investigate miRNA-122 (miR-122) and mir-RNA-199a (miR-199a) as a biomarker for predicting therapeutic efficacy in hepatitis C (HepC) patients. A total of 47 HepC 1b patients and 16 healthy subjects were enrolled in the study. Serum and exosomal mir-RNAs and other conventional biomarkers reflecting liver function were evaluated. The miR-122 levels in serum (miR-122 ) and exosomes (miR-122 ) were significantly lower in the Hepatitis C virus (HCV) genotype 1b patients than in the normal controls, but these levels were higher compared to the non-genotype 1b group. The mean miR-122 level in the sustained virological response (SVR) group was significantly higher than that in the non-response (NR) group (P < 0.01), and the miR-122 level in the SVR group was also higher than that in the NR group (P > 0.05), although this difference was not significant. miR-199a levels showed similar trends with the miR-122 levels in serum and exosomes. HCV RNA was negatively correlated with the miR-122 (r = -0.473, P = 0.004) and miR-122 (r = -0.424, P = 0.009) levels. miR-122 levels were positively associated with miR-199a levels (r = 0.453, P = 0.002) Univariate and multivariate regression analyses reveal that the miR-122 levels and ALT/AST ratio demonstrated a predictive value in evaluating patient outcomes. Serum miR-122 and miR-199a are potential biomarkers that reflect therapeutic efficacy.
微小 RNA（miRNA）已被证明与肝功能相关，被提出作为反映肝损伤的新生物标志物。本研究旨在探讨 miRNA-122（miR-122）和 miRNA-199a（miR-199a）作为丙型肝炎（HepC）患者治疗效果预测的生物标志物。共纳入 47 例 HepC 1b 患者和 16 例健康对照者。评估血清和外泌体 miRNA 及其他反映肝功能的常规生物标志物。HepC 基因型 1b 患者血清（miR-122）和外泌体（miR-122）miR-122 水平明显低于正常对照组，但高于非 1b 型组。持续病毒学应答（SVR）组的平均 miR-122 水平明显高于无应答（NR）组（P<0.01），SVR 组的 miR-122 水平也高于 NR 组（P>0.05），尽管差异无统计学意义。miR-199a 水平在血清和外泌体中与 miR-122 水平呈相似趋势。HCV RNA 与 miR-122（r=-0.473，P=0.004）和 miR-122（r=-0.424，P=0.009）水平呈负相关。miR-122 水平与 miR-199a 水平呈正相关（r=0.453，P=0.002）。单因素和多因素回归分析显示，miR-122 水平和 ALT/AST 比值在评估患者结局方面具有预测价值。血清 miR-122 和 miR-199a 是反映治疗效果的潜在生物标志物。
