Gao Yi Hui, Wu Lei, Wang Rui, Guo Qian, Chen Yi Ni, Hu Bing, Jiang Li Xin
Department of Ultrasonography, Shanghai Jiaotong University Affiliated No. 6 Hospital, Shanghai 200233, PR China.
Shanghai Institute of Ultrasound in Medicine, Shanghai 200233, PR China.
Oncotarget. 2017 Jun 6;8(23):37584-37593. doi: 10.18632/oncotarget.16621.
We evaluated the efficacy of contrast-enhanced ultrasound for assessing tumors after irradiation with sub-threshold focused ultrasound (FUS) ablation in pancreatic cancer xenografts in nude mice. Thirty tumor-bearing nude mice were divided into three groups: Group A received sham irradiation, Group B received a moderate-acoustic energy dose (sub-threshold), and Group C received a high-acoustic energy dose. In Group B, B-mode ultrasound (US), color Doppler US, and dynamic contrast-enhanced ultrasound (DCE-US) studies were conducted before and after irradiation. After irradiation, tumor growth was inhibited in Group B, and the tumors shrank in Group C. In Group A, the tumor sizes were unchanged. In Group B, contrast-enhanced ultrasound (CEUS) images showed a rapid rush of contrast agent into and out of tumors before irradiation. After irradiation, CEUS revealed contrast agent perfusion only at the tumor periphery and irregular, un-perfused volumes of contrast agent within the tumors. DCE-US perfusion parameters, including peak intensity (PI) and area under the curve (AUC), had decreased 24 hours after irradiation. PI and AUC were increased 48 hours and 2weeks after irradiation. Time to peak (TP) and sharpness were increased 24 hours after irradiation. TP decreased at 48 hours and 2 weeks after irradiation. CEUS is thus an effective method for early evaluation after irradiation with sub-threshold FUS.
我们评估了超声造影在评估裸鼠胰腺癌异种移植瘤经亚阈值聚焦超声（FUS）消融照射后肿瘤情况中的疗效。30只荷瘤裸鼠被分为三组：A组接受假照射，B组接受中等声能剂量（亚阈值）照射，C组接受高声能剂量照射。在B组中，照射前后分别进行了B型超声（US）、彩色多普勒超声及动态对比增强超声（DCE-US）检查。照射后，B组肿瘤生长受到抑制，C组肿瘤缩小，A组肿瘤大小无变化。在B组中，超声造影（CEUS）图像显示照射前造影剂快速进出肿瘤。照射后，CEUS显示造影剂仅在肿瘤周边灌注，肿瘤内部有不规则的未灌注造影剂区域。照射后24小时，DCE-US灌注参数，包括峰值强度（PI）和曲线下面积（AUC）降低。照射后48小时和2周，PI和AUC升高。照射后24小时，达峰时间（TP）和锐利度增加。照射后48小时和2周，TP降低。因此，CEUS是亚阈值FUS照射后早期评估的有效方法。
