Kim K E, Gordon D F, Maurer R A
Department of Physiology and Biophysics, University of Iowa, Iowa City 52242.
DNA. 1988 May;7(4):227-33. doi: 10.1089/dna.1988.7.227.
The gene for the beta-subunit of follicle-stimulating hormone (FSH-beta) was isolated from a library of bovine DNA fragments cloned in bacteriophage gamma and the complete nucleotide sequence of the gene was determined. The bovine FSH-beta gene contains approximately 4000 nucleotides and consists of three exons separated by two intervening sequences. The transcription initiation site of the gene was mapped by nuclease protection experiments. Analysis of RNA species present in pituitary mRNA demonstrated the presence of a 4.0-kb RNA containing FSH-beta sequences, which is the appropriate size for the primary transcript of the gene. Comparison of nucleotide sequence of the 5'-flanking sequence of the FSH-beta gene to the 5'-flanking regions of other pituitary glycoprotein hormone genes reveals little sequence similarity.
从克隆于噬菌体γ的牛DNA片段文库中分离出促卵泡激素β亚基（FSH-β）的基因，并测定了该基因的完整核苷酸序列。牛FSH-β基因约含4000个核苷酸，由三个外显子和两个间隔序列组成。通过核酸酶保护实验确定了该基因的转录起始位点。对垂体mRNA中存在的RNA种类进行分析，结果表明存在一种含有FSH-β序列的4.0 kb RNA，其大小与该基因的初级转录本相符。将FSH-β基因5'侧翼序列的核苷酸序列与其他垂体糖蛋白激素基因的5'侧翼区域进行比较，发现序列相似性很低。
