Dekel N, Lewysohn O, Ayalon D, Hazum E
Department of Hormone Research, Weizmann Institute of Science, Rehovot, Israel.
Endocrinology. 1988 Aug;123(2):1205-7. doi: 10.1210/endo-123-2-1205.
Specific receptors for gonadotropin releasing hormone (GnRH) in the rat oocyte have been identified by using two independent methods. Light microscopic autoradiography, utilizing an iodinated biologically active photoaffinity derivative of GnRH, revealed specific binding of the neurohormone to rat oocytes. Furthermore, the presence of GnRH-receptor is also evident from indirect fluorescent immunocytochemistry that shows binding of GnRH-receptor antibodies to rat oocytes which is neither detected with non immune serum nor with antiserum depleted of GnRH-receptor antibodies. These antibodies to the GnRH-receptor, also bind to both cumulus and granulosa cells but not to rat basophilic leukemia cells. The presence of specific GnRH receptors on rat oocytes provides an experimental basis for understanding the molecular events involved in GnRH-induced oocyte maturation.
通过两种独立的方法已鉴定出大鼠卵母细胞中促性腺激素释放激素（GnRH）的特异性受体。利用GnRH的碘化生物活性光亲和衍生物进行的光学显微镜放射自显影显示，该神经激素与大鼠卵母细胞有特异性结合。此外，GnRH受体的存在从间接荧光免疫细胞化学中也很明显，该方法显示GnRH受体抗体与大鼠卵母细胞结合，而在非免疫血清或去除GnRH受体抗体的抗血清中均未检测到这种结合。这些针对GnRH受体的抗体也与卵丘细胞和颗粒细胞结合，但不与大鼠嗜碱性白血病细胞结合。大鼠卵母细胞上特异性GnRH受体的存在为理解GnRH诱导卵母细胞成熟所涉及的分子事件提供了实验基础。
