促黄体生成素释放激素（gonadotropin-releasing hormone）预处理可提高 129 品系小鼠超排卵的体外受精率。

In vitro fertility rate of 129 strain is improved by buserelin (gonadotropin-releasing hormone) administration prior to superovulation.

机构信息

Assisted Reproduction Technologies and Cryopreservation Unit, CMB, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland, USA.

出版信息

Lab Anim. 2012 Oct;46(4):299-303. doi: 10.1258/la.2012.012073.

DOI:10.1258/la.2012.012073

PMID:23097563

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3500660/

Abstract

The 129 mice are well recognized for their low fertility and it is speculated that this lack of fertility may be due to the oocyte condition. In this study we investigated superovulation regimens for the 129S1/SvImJ mouse strain to improve the oocyte quality and fertility rate of in vitro fertilization (IVF). Female mice were divided into four groups based on hormone and timing of injection. Group 1 received pregnant mare serum gonadotropin (PMSG) and 48 h later human chorionic gonadotropin (hCG); using the same dose, group 2 received hCG 52 h post-PMSG and group 3, 55 h post-PMSG. Group 4 received buserelin (gonadotropin-releasing hormone agonist [GnRH]) followed 24 h later by PMSG and then hCG 55 h post-PMSG. IVF was performed using 129S1/SvImJ oocytes and sperm; C57BL/6J sperm with 129S1/SvImJ oocytes was used as fertility control. The IVF fertility rate was 1% (Groups 1 and 2), 17% (Group 3) and 55% (Group 4) for 129 oocytes fertilized with 129 sperm. For 129 oocytes fertilized with C57BL/6J sperm, the fertility rate was 5% (Group 1), 10% (Group 2), 40% (Group 3) and 59% (Group 4). These results suggest that extending the interval time between PMSG and hCG and giving GnRH in addition to the standard PMSG and hCG treatments can improve IVF fertility rate of 129S1/SvImJ mouse strains significantly.

摘要

129 小鼠以其低生育率而闻名，据推测，这种生育能力低下可能是由于卵母细胞的状态所致。在这项研究中，我们研究了超排卵方案对 129S1/SvImJ 小鼠品系的影响，以提高体外受精（IVF）的卵母细胞质量和受精率。将雌性小鼠根据激素和注射时间分为四组。第 1 组接受孕马血清促性腺激素（PMSG），48 小时后给予人绒毛膜促性腺激素（hCG）；第 2 组以相同剂量，PMSG 后 52 小时给予 hCG，第 3 组 PMSG 后 55 小时给予 hCG。第 4 组接受布舍瑞林（促性腺激素释放激素激动剂[GnRH]），24 小时后给予 PMSG，然后 PMSG 后 55 小时给予 hCG。使用 129S1/SvImJ 卵母细胞和精子进行 IVF；用 129S1/SvImJ 卵母细胞受精的 C57BL/6J 精子作为生育力对照。用 129 精子受精的 129 卵母细胞的 IVF 受精率为 1%（第 1 和第 2 组）、17%（第 3 组）和 55%（第 4 组）。用 C57BL/6J 精子受精的 129 卵母细胞的受精率为 5%（第 1 组）、10%（第 2 组）、40%（第 3 组）和 59%（第 4 组）。这些结果表明，延长 PMSG 和 hCG 之间的间隔时间，并在标准 PMSG 和 hCG 治疗的基础上给予 GnRH，可显著提高 129S1/SvImJ 小鼠品系的 IVF 受精率。