Gonzalez-Pacanowska D, Marco C, Iglesias J, Garcia-Martinez J, Garcia-Peregrin E
Department of Biochemistry, University of Granada, Spain.
Enzyme. 1988;39(2):90-4.
Phosphorylation and decarboxylation of mevalonic acid have been measured in different tissues of chicks fed a cholestyramine diet from hatching until 18 days of age. Hepatic and intestinal phosphorylation and decarboxylation of mevalonate were slightly although significantly increased from 15 days of treatment. Direct measurements of 5-pyrophosphomevalonate decarboxylase activity using the specific substrate of this enzyme corroborated these data. Brain enzymatic activities remained unaltered with respect to controls. These results suggest that enzymes responsible for the conversion of mevalonate to isopentenyl pyrophosphate from neonatal chick liver and intestine alter their activities in a coordinate fashion and may play an important role in the regulation of cholesterogenesis in these tissues.
从孵化到18日龄期间，用消胆胺日粮喂养雏鸡，测定了其不同组织中甲羟戊酸的磷酸化和脱羧作用。从治疗第15天起，肝脏和肠道中甲羟戊酸的磷酸化和脱羧作用略有增加，但具有显著差异。使用该酶的特异性底物直接测量5-焦磷酸甲羟戊酸脱羧酶活性证实了这些数据。与对照组相比，脑酶活性没有变化。这些结果表明，新生雏鸡肝脏和肠道中负责将甲羟戊酸转化为异戊烯基焦磷酸的酶以协调的方式改变其活性，可能在这些组织中胆固醇生物合成的调节中起重要作用。
