Bonito Gregory, Hameed Khalid, Toome-Heller Merje, Healy Rosanne, Reid Chantal, Liao Hui-Ling, Aime M Catherine, Schadt Christopher, Vilgalys Rytas
a Department of Plant Soil and Microbial Sciences , Michigan State University , East Lansing , Michigan 48824.
b Biology Department , Duke University , Durham , North Carolina 27708.
Mycologia. 2017;109(1):18-26. doi: 10.1080/00275514.2016.1271689. Epub 2017 Jan 9.
Among fungi isolated from healthy root mycobiomes of Populus, we discovered a new endorrhizal fungal species belonging to the rust lineage Pucciniomycotina, described here as Atractiella rhizophila. We characterized this species by transmission electron microscopy (TEM), phylogenetic analysis, and plant bioassay experiments. Phylogenetic sequence analysis of isolates and available environmental and reference sequences indicates that this new species, A. rhizophila, has a broad geographic and host range. Atractiella rhizophila appears to be present in North America, Australia, Asia, and Africa and is associated with trees, orchids, and other agriculturally important species, including soybean, corn, and rice. Despite the large geographic and host range of this species sampling, A. rhizophila appears to have exceptionally low sequence variation within nuclear rDNA markers examined. With inoculation studies, we demonstrate that A. rhizophila is nonpathogenic, asymptomatically colonizes plant roots, and appears to foster plant growth and elevated photosynthesis rates.
在从杨树健康根系真菌群落中分离出的真菌中，我们发现了一种属于锈菌谱系柄锈菌亚门的新的内生真菌物种，在此将其描述为嗜根白术菌。我们通过透射电子显微镜（TEM）、系统发育分析和植物生物测定实验对该物种进行了表征。对分离株以及可用的环境序列和参考序列进行的系统发育序列分析表明，这个新物种嗜根白术菌具有广泛的地理分布和宿主范围。嗜根白术菌似乎存在于北美、澳大利亚、亚洲和非洲，并且与树木、兰花以及其他农业重要物种相关，包括大豆、玉米和水稻。尽管该物种采样的地理分布和宿主范围很广，但在所检测的核rDNA标记内，嗜根白术菌的序列变异似乎极低。通过接种研究，我们证明嗜根白术菌无致病性，能无症状地定殖于植物根部，并且似乎能促进植物生长和提高光合作用速率。
